TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| TOP10 | Cloning strain | Invitrogen |
| INV110 | dam and dcm methylase deficient | Invitrogen |
| M. morganii | ||
| G858 | Clinical isolate, tigecycline MIC = 1 μg/ml | This study |
| G1492 | Clinical isolate, tigecycline MIC = 4 μg/ml | This study |
| GC7676 | G1492 insertion mutant, EZ::TN inserted in 453 bp of acrA | This study |
| GC7677 | G1492 insertion mutant, EZ::TN inserted in 946 bp of acrA | This study |
| GC7743 | GC7676 transformed with pCLL3444 | This study |
| GC7744 | GC7677 transformed with pCLL3444 | This study |
| Plasmids | ||
| pCR2.1-TOPO | PCR cloning vector | Invitrogen |
| pUCGm | pUC19 derivative containing gentamicin resistance cassette | 13 |
| pCLL3443 | pCR2.1-TOPO with cloned acrAB genes (4,859-bp PCR fragment) | This study |
| pCLL3444 | pCLL3443 with cloned gentamicin cassette (878-bp XbaI fragment) | This study |