TABLE 3.

Primers and fluorescent probes used for PCR and RT-PCR

Gene(s)	Product size (bp)	Purpose	Sequence (5′→3′) of:
			Forward primer	Reverse primer	Fluorescent probea
acrAB	4,859	TOPO cloning	ATCCACTAATGACGTCGC	GCAGAGTAAATGCCTGAACG	NAb
acrA	72	RT-PCR	ACCTGCGTCTGAAACAAGAAATC	CCAGGCTGACAGCAACTTTACC	CCAGCGGTGCCGTGGATAAAGAAC
rrsE	71	RT-PCR	TTGACGTTACCCGCAGAAGAA	GCTTGCACCCTCCGTATTACC	TAACTCCGTGCCAGCAGCCG

^aLabeled with 6-carboxyfluorescein (6′-FAM) at the 5′ end and with 6-carboxytetramethylrodamine (TAMRA) at the 3′ end.

^bNA, not applicable.