Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1968 Jan;16(1):33–38. doi: 10.1128/am.16.1.33-38.1968

Morphological and Biochemical Differentiation of Achromobacter and Moraxella (Debord's Tribe Mimeae)1

Gerald L Gilardi 1
PMCID: PMC547302  PMID: 5636469

Abstract

To determine the most useful laboratory tests for the differentiation of Achromobacter anitratus, Achromobacter lwoffii, and Moraxella duplex (DeBord's tribe Mimeae), 157 strains of these bacteria, isolated from clinical specimens, were examined for their morphological and biochemical characteristics. There were several differences between these nonfermentative, gram-negative diplococci: Moraxella was nonglucolytic in either infusion base or synthetic base, oxidase-positive, and sensitive to penicillin, whereas Achromobacter produced variable carbohydrate activity, and was oxidase-negative and resistant to penicillin. A. anitratus was distinguished from A. lwoffii in that the former utilized infusion media containing either glucose or 10% lactose, whereas the latter did not. Both species utilized the same carbohydrates in a chemically defined medium, although the latter acted more sluggishly.

Full text

PDF
33

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEER H. ZUR DIAGNOSTIK GRAMNEGATIVER, AEROBER STAEBCHEN. Pathol Microbiol (Basel) 1963;26:607–634. [PubMed] [Google Scholar]
  2. BILLING E. Studies on a soap tolerant organism: a new variety of Bacterium anitratum. J Gen Microbiol. 1955 Oct;13(2):252–260. doi: 10.1099/00221287-13-2-252. [DOI] [PubMed] [Google Scholar]
  3. BRISOU J., MORICHAU-BEAUCHANT R. Identité biochimique entre certaines souches de B. anitratum et Moraxella lwoffi. Ann Inst Pasteur (Paris) 1952 May;82(5):640–643. [PubMed] [Google Scholar]
  4. BRISOU J., PREVOT A. R. Etudes de systématique bactérienne. X. Révision des especes réunies dans le genre Achromobacter. Ann Inst Pasteur (Paris) 1954 Jun;86(6):722–728. [PubMed] [Google Scholar]
  5. COWAN S. T., STEEL K. J. Diagnostic tables for the common medical bacteria. J Hyg (Lond) 1961 Sep;59:357–372. doi: 10.1017/s0022172400039024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Controni G., Stewart J., Jones R. Lysine decarboxylase studies with Herellea. Am J Med Technol. 1965 Nov-Dec;31(6):421–424. [PubMed] [Google Scholar]
  7. ELLIS E. M. The recovery of Bacterium anitraum (B5w) from animals. Am J Vet Res. 1961 May;22:610–613. [PubMed] [Google Scholar]
  8. Elrod R. P., Braun A. C. Pseudomonas aeruginosa: Its Rôle as a Plant Pathogen. J Bacteriol. 1942 Dec;44(6):633–645. doi: 10.1128/jb.44.6.633-645.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Elston H. R., Hoffman K. C. Identification and clinical significance of Achromobacter (Herellea) anitratus in urinary tract infections. Am J Med Sci. 1966 Jan;251(1):75–80. doi: 10.1097/00000441-196601000-00013. [DOI] [PubMed] [Google Scholar]
  10. Gilardi G. L. Achromobacter and Moraxella (tribe Mimeae): a review and new studies. Am J Med Technol. 1967 May-Jun;33(3):201–220. [PubMed] [Google Scholar]
  11. HALVORSEN J. F. GLIDING MOTILITY IN THE ORGANISMS BACTERIUM ANITRATUM (B5W), MORAXELLA LWOFFI AND ALKALIGENES HAEMOLYSANS, AS COMPARED TO MORAXELLA NONLIQUEFACIENS. Acta Pathol Microbiol Scand. 1963;59:200–204. doi: 10.1111/j.1699-0463.1963.tb01789.x. [DOI] [PubMed] [Google Scholar]
  12. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Henderson A. The Moraxella iwoffi group of bacteria; a review. Antonie Van Leeuwenhoek. 1965;31(4):395–413. doi: 10.1007/BF02045919. [DOI] [PubMed] [Google Scholar]
  14. Jyssum K., Joner P. E. Regulation of the nitrogen assimilation from nitrate and nitrite in Bacterium anitratum (B5W). Acta Pathol Microbiol Scand. 1965;64(3):387–397. doi: 10.1111/apm.1965.64.3.387. [DOI] [PubMed] [Google Scholar]
  15. KABLER P. W., KENNER B. A. Members of the tribe Mimeae isolated from river water. J Bacteriol. 1956 Dec;72(6):870–870. doi: 10.1128/jb.72.6.870-870.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KLINGE K. [On the systematology of Bacterium anitratum, Diplococcus mucosus and Moraxella lwoffi. (Genus Acinetobacter according to Brisou and Prevot)]. Arch Hyg Bakteriol. 1959 Dec;143:587–606. [PubMed] [Google Scholar]
  17. LEMOIGNE M., GIRARD H., JACOBELLI G. Bactérie du sol utilisant facilement le 2-3 butanediol. Ann Inst Pasteur (Paris) 1952 Apr;82(4):389–398. [PubMed] [Google Scholar]
  18. MØLLER V. Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta Pathol Microbiol Scand. 1955;36(2):158–172. doi: 10.1111/j.1699-0463.1955.tb04583.x. [DOI] [PubMed] [Google Scholar]
  19. Nelson J. D., Shelton S. Cultural, biochemical, and immunological properties of Mima, Herellea, and Flavobacterium species. Appl Microbiol. 1965 Sep;13(5):801–807. doi: 10.1128/am.13.5.801-807.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. OPSAHL R., Jr Bacterium anitratum (Acinetobacter anitratum) isolated from a case with cerebral abscess and purulent meningitis. Acta Pathol Microbiol Scand. 1961;51:72–74. doi: 10.1111/j.1699-0463.1961.tb00344.x. [DOI] [PubMed] [Google Scholar]
  21. PICKETT M. J., MANCLARK C. R. TRIBE MIMEAE: AN ILLEGITIMATE EPITHET. Am J Clin Pathol. 1965 Feb;43:161–165. doi: 10.1093/ajcp/43.2.161. [DOI] [PubMed] [Google Scholar]
  22. PIECHAUD M. [Motility of the Moraxella]. Ann Inst Pasteur (Paris) 1963 Feb;104:291–297. [PubMed] [Google Scholar]
  23. PIKE R. M., SCHULZE M. L., McCULLOUGH M. Isolation of Mima polymorpha from a patient with subacute bacterial endocarditis. Am J Clin Pathol. 1951 Nov;21(11):1094–1096. doi: 10.1093/ajcp/21.11_ts.1094. [DOI] [PubMed] [Google Scholar]
  24. SELLERS W. MEDIUM FOR DIFFERENTIATING THE GRAM-NEGATIVE, NONFERMENTING BACILLI OF MEDICAL INTEREST. J Bacteriol. 1964 Jan;87:46–48. doi: 10.1128/jb.87.1.46-48.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schaub I. G., Hauber F. D. A Biochemical and Serological Study of a Group of Identical Unidentifiable Gram-negative Bacilli from Human Sources. J Bacteriol. 1948 Oct;56(4):379–385. doi: 10.1128/jb.56.4.379-385.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. THOMPSON R. B., COUEY B. J. A relatively unknown pathogen--Mimeae. Am J Med Technol. 1962 Jan-Feb;28:64–66. [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES