Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1968 Jan;16(1):69–72. doi: 10.1128/am.16.1.69-72.1968

Continuous Production of Clostridium tetani Toxin

Bengt Zacharias 1, Marianne Björklund 1
PMCID: PMC547313  PMID: 4865906

Abstract

The continuous production of Clostridium tetani toxin has been carried out in a 1-liter stirred culture vessel for as long as 65 days. Toxin production of approximately 120 flocculating units per ml was maintained with a dilution rate of 0.125 hr-1, a temperature of 34 C, a pH of 7.4, and the addition to the medium of 0.1 g of potassium chloride per liter. The average minimal lethal intraperitoneal dose of the toxin in mice was approximately 106 per ml.

Full text

PDF
69

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Feeney R. E., Mueller J. H., Miller P. A. Growth requirements of clostridium tetani: II. Factors exhausted by growth of the organism. J Bacteriol. 1943 Dec;46(6):559–562. doi: 10.1128/jb.46.6.559-562.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HULTMAN E. Rapid specific method for determination of aldosaccharides in body fluids. Nature. 1959 Jan 10;183(4654):108–109. doi: 10.1038/183108a0. [DOI] [PubMed] [Google Scholar]
  3. KOCH W., KAPLAN D. A simple method for obtaining highly potent tetanus toxin. J Immunol. 1953 Jan;70(1):1–5. [PubMed] [Google Scholar]
  4. LATHAM W. C., BENT D. F., LEVINE L. Tetanus toxin production in the absence of protein. Appl Microbiol. 1962 Mar;10:146–152. doi: 10.1128/am.10.2.146-152.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MUELLER J. H., MILLER P. A. Variable factors influencing the production of tetanus toxin. J Bacteriol. 1954 Mar;67(3):271–277. doi: 10.1128/jb.67.3.271-277.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nielsen P. A. Large-scale production of tetanus toxoid. Appl Microbiol. 1967 Mar;15(2):453–454. doi: 10.1128/am.15.2.453-454.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. RAYNAUD M. Extraction de la toxine tétanique a partir des corps microbiens. Ann Inst Pasteur (Paris) 1951 Apr;80(4):356–377. [PubMed] [Google Scholar]
  8. REDEI A., NAGY S. Simultaneous determination of dextran and glucose in serum. Nature. 1961 Jul 8;191:173–174. doi: 10.1038/191173a0. [DOI] [PubMed] [Google Scholar]
  9. THOMSON R. O. A semi-continuous method for the large-scale production of tetanus toxin. Nature. 1957 Nov 23;180(4595):1126–1127. doi: 10.1038/1801126a0. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES