Table 1.
Gene | Dir | Primer Sequence | Length (bp) | Product length (bp) | Efficiency (%) |
---|---|---|---|---|---|
GAPDH | F | 5′-CCATGAGAAGTATGACAAC-3′ | 19 | 115 | 98.3 |
R | 5′-GAGTCCTTCCACGATACC-3′ | 18 | |||
STAT3 (Var. 1,2,3) | F | 5′-AGCAGGAGGGCAGTTTGAGTC-3′ | 21 | 241 | 99.1 |
R | 5′-TTTAAAAGTGCCCAGATTGCTC-3′ | 22 | |||
STAT5A | F | 5′- ACATGTACCCACAGAACCCTGACC-3′ | 24 | 239 | 98.2 |
R | 5′- CACAACACGACCGCTTCACATTGC-3′ | 24 |