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Applied Microbiology logoLink to Applied Microbiology
. 1968 Feb;16(2):267–270. doi: 10.1128/am.16.2.267-270.1968

Influence of Catalase Activity on Resistance of Coagulase-positive Staphylococci to Hydrogen Peroxide1

V M Amin 1, N F Olson 1
PMCID: PMC547393  PMID: 5645413

Abstract

Catalase activities of intact cells and cell-free extracts of coagulase-positive staphylococcal cultures 105B and 558D isolated from milk, culture 25042 from a clinical source, and Staphylococcus aureus 196E were determined at 32.2 C. Cultures were treated with 0.025 and 0.05% hydrogen peroxide at 37.8 and 54.4 C and without hydrogen peroxide at 54.4 C to determine the relationship between catalase activity and resistance to these treatments. The relationship held true for cultures 105B and 196E; culture 105B had the lowest catalase activity and lowest resistance to H2O2 at 37.8 C, whereas S. aureus 196E possessed a high catalase activity and was most resistant at 37.8 C. Catalase activities of cell-free extracts of cultures 25042, 558, and 196E were similar, but resistance to H2O2 at 37.8 C was greater for culture 196E. The lower resistance of culture 25042 was related to low catalase activities of whole cells of this culture, which were only one-third that of whole cells of culture 196E. Culture 558 was least resistant to heat treatment at 54.4 C and showed the greatest sensitivity to added H2O2 at this temperature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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