Abstract
Spores of Aspergillus ochraceus and Septomyxa affinis were produced on a large scale by surface sporulation on moist wheat bran and barley. 11α-Hydroxylation of progesterone and Reichstein's compound S by spores of A. ochraceus and 1-dehydrogenation of compound S by spores of S. affinis were carried out in 5-liter fermentors. It was shown that, above a certain minimum, increase in aeration and agitation did not significantly affect steroid conversion. The industrial feasibility of the spore process was further demonstrated by 11α-hydroxylation of 6α-fluoro-16α,17α-dihydroxypregn-4-ene-3,20-dione in a modified 200-gal stainless-steel vessel with spores of A. ochraceus. Strict aseptic conditions are not necessary, either during harvesting of spores or during steroid transformation.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BURTON R. B., ZAFFARONI, KEUTMANN E. H. Paper chromatography of steroids. II. Corticosteroids and related compounds. J Biol Chem. 1951 Feb;188(2):763–771. [PubMed] [Google Scholar]
- SINGH K., SEHGAL S. N., VEZINA C. TRANSFORMATION OF REICHSTEIN'S COMPOUND 'S' AND OXIDATION OF CARBOHYDRATES BY SPORES OF SEPTOMYXA AFFINIS. Can J Microbiol. 1965 Apr;11:351–364. doi: 10.1139/m65-043. [DOI] [PubMed] [Google Scholar]
- VEZINA C., SEHGAL S. N., SINGH K. Transformation of steroids by spores of microorganisms. I. Hydroxylation of progesterone by conidia of Aspergillus ochraceus. Appl Microbiol. 1963 Jan;11:50–57. doi: 10.1128/am.11.1.50-57.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]