Abstract
A new mass-culturing technique for synchronized cells is described. Continuous dilution with fresh culture medium provides the cells with nearly constant environmental conditions (continuous resupply of nutrients, constant adjustment of pH, maintenance of a nearly constant cell mass-to-culture volume ratio, etc.) at high cell densities, thereby resulting in the yield of ample cellular material for most biochemical studies during the cell cycle. The new mass-culturing system, when utilized to culture synchronous Chlorella pyrenoidosa, yielded approximately three times as much cellular material as a previous nondilution method, in the same time period and in less than one-tenth the culture volume. Although the newly developed mass-culture method was tested with a unicellular green alga, the basic method should be readily adaptable to synchronous cultures of other microorganisms and to cells in tissue culture.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HARE T. A., SCHMIDT R. R. NITROGEN METABOLISM DURING SYNCHRONOUS GROWTH OF CHLORELLA PYRENOIDOSA. I. PROTEIN AMINO ACID DISTRIBUTION. J Cell Physiol. 1965 Feb;65:63–67. doi: 10.1002/jcp.1030650109. [DOI] [PubMed] [Google Scholar]
- JOHNSON R. A., SCHMIDT R. R. INTRACELLULAR DISTRIBUTION OF SULFUR DURING THE SYNCHRONOUS GROWTH OF CHLORELLA PYRENOIDOSA. Biochim Biophys Acta. 1963 Aug 13;74:428–437. doi: 10.1016/0006-3002(63)91386-6. [DOI] [PubMed] [Google Scholar]
- SCHERBAUM O. H. Synchronous division of microorganisms. Annu Rev Microbiol. 1960;14:283–310. doi: 10.1146/annurev.mi.14.100160.001435. [DOI] [PubMed] [Google Scholar]
- SCHMIDT R. R., KING K. W. Metabolic shifts during synchronous growth of Chlorella pyrenoidosa. Biochim Biophys Acta. 1961 Feb 18;47:391–392. doi: 10.1016/0006-3002(61)90300-6. [DOI] [PubMed] [Google Scholar]
- SCHMIDT R. R. Nitrogen and phosphorus metabolism during synchronous growth of Chlorella pyrenoidosa. Exp Cell Res. 1961 Mar;23:209–217. doi: 10.1016/0014-4827(61)90031-3. [DOI] [PubMed] [Google Scholar]
- SCHMIDT R. R., SPENCER H. T. STEADY-STATE AND SYNCHRONOUS GROWTH OF CHLORELLA PYRENOIDOSA. J Cell Physiol. 1964 Oct;64:249–255. doi: 10.1002/jcp.1030640209. [DOI] [PubMed] [Google Scholar]
- SOROKIN C., MYERS J. A high-temperature strain of Chlorella. Science. 1953 Mar 27;117(3039):330–331. doi: 10.1126/science.117.3039.330. [DOI] [PubMed] [Google Scholar]