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. 1968 Mar;16(3):509–518. doi: 10.1128/am.16.3.509-518.1968

Secondary and Apparent Primary Antibody Responses After Group A Streptococcal Vaccination of 21 Children

Benedict F Massell 1,2, J Gabriel Michael 1,2, Jacqueline Amezcua 1,2, Myron Siner 1,2
PMCID: PMC547450  PMID: 4870220

Abstract

A partially purified M protein, extracted from a mouse-virulent strain of type 3, group A streptococcus, was administered subcutaneously in gradually increasing amounts at weekly intervals to 21 children in a Family Program. Seven children with type 3 bactericidal antibody in prevaccination sera showed a secondary response. Of 14 children with no detectable type 3 bactericidal antibody prior to vaccination, 13 developed definite type 3 antibody during or soon after vaccination. This response appeared to be of the primary variety in at least some of the 13 children because (i) the total incidence of antibody response (20 of 21) was greater than can be accounted for by the documented incidence of clinical and subclinical type 3 infections among children of our Family Program during a period of 14 years, (ii) the response in the 13 children with no detectable antibody prior to vaccination was more delayed than in those showing a definite secondary response, and (iii) on the average, the amount of vaccine needed for a response in these 13 children was 15 to 28 times greater than that required for the secondary response. Local skin reactions were variable among the vaccinated children. Systemic reactions were infrequent and not severe. The giving of multiple injections of partially purified M protein did not seem to cause cutaneous hypersensitivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DENNY F. W., Jr, THOMAS L. The demonstration of type specific streptococcal antibody by a hemagglutination technique employing tannic acid. J Clin Invest. 1953 Nov;32(11):1085–1093. doi: 10.1172/JCI102831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FOX E. N. ANTIGENICITY OF THE M PROTEINS OF GROUP A HEMOLYTIC STREPTOCOCCI. J Immunol. 1964 Nov;93:826–837. [PubMed] [Google Scholar]
  3. Fox E. N., Wittner M. K. Antigenicity of the M proteins of group A hemolytic streptococci. II. Antibody response in rabbits to vaccines prepared with oil emulsions and aluminum hydroxide. J Immunol. 1966 Jul;97(1):86–94. [PubMed] [Google Scholar]
  4. Fox E. N., Wittner M. K., Dorfman A. Antigenicity of the M proteins of group A hemolytic streptococci. 3. Antibody responses and cutaneous hypersensitivity in humans. J Exp Med. 1966 Dec 1;124(6):1135–1151. doi: 10.1084/jem.124.6.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KANTOR F. S., COLE R. M. Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin. J Exp Med. 1960 Jul 1;112:77–96. doi: 10.1084/jem.112.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LANCEFIELD R. C. Current knowledge of type-specific M antigens of group A streptococci. J Immunol. 1962 Sep;89:307–313. [PubMed] [Google Scholar]
  7. LANCEFIELD R. C. Differentiation of group A streptococci with a common R antigen into three serological types, with special reference to the bactericidal test. J Exp Med. 1957 Oct 1;106(4):525–544. doi: 10.1084/jem.106.4.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LANCEFIELD R. C., PERLMANN G. E. Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus. J Exp Med. 1952 Jul;96(1):71–82. doi: 10.1084/jem.96.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MILLER J. M., STANCER S. L., MASSELL B. F. A controlled study of beta hemolytic streptococcal infection in rheumatic families. I. Streptococcal disease among healthy siblings. Am J Med. 1958 Dec;25(6):825–844. doi: 10.1016/0002-9343(58)90056-1. [DOI] [PubMed] [Google Scholar]
  10. POTTER E. V., STOLLERMAN G. H., SIEGEL A. C. Recall of type specific antibodies in man by injections of streptococcal cell walls. J Clin Invest. 1962 Feb;41:301–310. doi: 10.1172/JCI104483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SCHMIDT W. C. Type-specific antibody formation in man following injection of streptococcal M protein. J Infect Dis. 1960 May-Jun;106:250–255. doi: 10.1093/infdis/106.3.250. [DOI] [PubMed] [Google Scholar]
  12. STOLLERMAN G. H., KANTOR F. S., GORDON B. D. Accessory plasma factors involved in the bactericidal test for type-specific antibody to group A streptococci. I. A typical behavior of some human and rabbit bloods. J Exp Med. 1958 Oct 1;108(4):475–491. doi: 10.1084/jem.108.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WOLFE C. K., Jr, HAYASHI J. A., WALSH G., BARKULIS S. S. Type-specific antibody response in man to injections of cell walls and M protein from group A, type 14 streptococci. J Lab Clin Med. 1963 Mar;61:459–468. [PubMed] [Google Scholar]

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