Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1968 Mar;16(3):524–527. doi: 10.1128/am.16.3.524-527.1968

Glycerol Ester Hydrolase Activity of Lactic Acid Bacteria

Anders Oterholm 1,1, Z John Ordal 1, Lloyd D Witter 1
PMCID: PMC547452  PMID: 5649866

Abstract

Seventeen strains of lactic acid bacteria were assayed for their glycerol ester hydrolase activity by using an improved agar-well technique, and eight strains by determining the activity in cell-free extracts using a pH-stat procedure. All cultures tested showed activity and hydrolyzed tributyrin more actively than they did tricaproin. The cell extract studies demonstrated that the cells contained intracellular esterases and lipases. The culture supernatant fluid was without activity. The lipase and the esterase differed in their relative activity to each other in the different extracts and in the ease by which they could be freed from the cellular debris. It is suggested that the lipase of these organisms is an endoenzyme and the esterase an ectoenzyme.

Full text

PDF
524

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aspen A. J., Wolin M. J. Solubilization and reconstitution of a particulate hydrogenase from Vibrio succinogenes. J Biol Chem. 1966 Sep 25;241(18):4152–4156. [PubMed] [Google Scholar]
  2. DESNUELLE P., CONSTANTIN M. J., BALDY J. Technique potentiométrique pour la measure de l'activité de la lipase pancréatique. Bull Soc Chim Biol (Paris) 1955;37(2-3):285–290. [PubMed] [Google Scholar]
  3. DESNUELLE P. Pancreatic lipase. Adv Enzymol Relat Subj Biochem. 1961;23:129–161. doi: 10.1002/9780470122686.ch4. [DOI] [PubMed] [Google Scholar]
  4. Hodgkiss W., Ordal Z. J., Cann D. C. The comparative morphology of the spores of Clostridium botulinum type E and the spores of the "OS mutant". Can J Microbiol. 1966 Dec;12(6):1283–1284. doi: 10.1139/m66-170. [DOI] [PubMed] [Google Scholar]
  5. IKAWA M. NATURE OF THE LIPIDS OF SOME LACTIC ACID BACTERIA. J Bacteriol. 1963 Apr;85:772–781. doi: 10.1128/jb.85.4.772-781.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kates M. Bacterial lipids. Adv Lipid Res. 1964;2:17–90. [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. PETERSON M. H., JOHNSON M. J. Delayed hydrolysis of butterfat by certain lactobacilli and micrococci isolated from cheese. J Bacteriol. 1949 Nov;58(5):701–708. doi: 10.1128/jb.58.5.701-708.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Parry R. M., Jr, Chandan R. C., Shahani K. M. Rapid and sensitive assay for milk lipase. J Dairy Sci. 1966 Apr;49(4):356–360. doi: 10.3168/jds.S0022-0302(66)87874-8. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES