Abstract
A method is described for the use of antiplague serum in Blood Agar Base plating media to detect fraction I-positive Pasteurella pestis. The antiserum was produced conveniently and in large volume in rabbits by use of Cutter plague vaccine combined with Freund's complete adjuvant. P. pestis colonies were specifically identified within 48 hr after plating by the presence of a precipitin ring surrounding each colony. The basis of the test was shown to be a precipitin reaction between fraction I antigen released from P. pestis colonies after chloroform vapor treatment and fraction I antibody present in the antiserum-agar medium.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAKER E. E., SOMMER H., FOSTER L. E., MEYER E., MEYER K. F. Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis. J Immunol. 1952 Feb;68(2):131–145. [PubMed] [Google Scholar]
- BALTAZARD M., DAVIS D. H., DEVIGNAT R., GIRARD G., GOHAR M. A., KARTMAN L., MEYER K. F., PARKER M. T., POLLITZER R., PRINCE F. M. Recommended laboratory methods for the diagnosis of plague. Bull World Health Organ. 1956;14(3):457–509. [PMC free article] [PubMed] [Google Scholar]
- BURROWS T. W. VIRULENCE OF PASTEURELLA PESTIS AND IMMUNITY TO PLAGUE. Ergeb Mikrobiol Immunitatsforsch Exp Ther. 1963;37:59–113. doi: 10.1007/978-3-662-36742-1_2. [DOI] [PubMed] [Google Scholar]
- CHEN T. H., LARSON A., MEYER K. F. STUDIES ON IMMUNIZATION AGAINST PLAGUE. XVII. EXPERIMENTAL STUDIES ON THE EFFECTIVENESS OF ALUMINUM-HYDROXIDE-ADSORBED FORMALIN-KILLED PASTEURELLA PESTIS. J Immunol. 1964 Oct;93:668–676. [PubMed] [Google Scholar]
- CHEN T. H., MEYER K. F. Studies on immunization against plague. VII. A hemagglutination test with the protein fraction of Pasteurella pestis: a serologic comparison of virulent and avirulent strains with observations on the structure of the bacterial cells and its relationship to infection and immunity. J Immunol. 1954 Apr;72(4):282–298. [PubMed] [Google Scholar]
- CHEN T. H., QUAN S. F., MEYER K. F. Studies on immunization against plague. II. The complement-fixation test. J Immunol. 1952 Feb;68(2):147–158. [PubMed] [Google Scholar]
- Chen T. H., Meyer K. F. An evaluation of Pasteurella pestis fraction-1-specific antibody for the confirmation of plague infections. Bull World Health Organ. 1966;34(6):911–918. [PMC free article] [PubMed] [Google Scholar]
- LARSON C. L., PHILIP C. B., WICHT W. C., HUGHES L. E. Precipitin reactions with soluble antigens from suspensions of Pasteurella pestis or from tissues of animals dead of plague. J Immunol. 1951 Oct;67(4):289–298. [PubMed] [Google Scholar]
- LAWTON W. D., FUKUI G. M., SURGALLA M. J. Studies on the antigens of Pasteurella pestis and Pasteurella pseudotuberculosis. J Immunol. 1960 May;84:475–479. [PubMed] [Google Scholar]
- MOODY M. D., WINTER C. C. Rapid identification of Pasteurella pestis with fluorescent antibody. III. Staining Pasteurella pestis in tissue impression smears. J Infect Dis. 1959 May-Jun;104(3):288–294. doi: 10.1093/infdis/104.3.288. [DOI] [PubMed] [Google Scholar]
- OUCHTERLONY O. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Pathol Microbiol Scand. 1953;32(2):230–240. [PubMed] [Google Scholar]
- QUAN S. F., KNAPP W., GOLDENBERG M. I., HUDSON B. W., LAWTON W. D., CHEN T. H., KARTMAN L. ISOLATION OF A STRAIN OF PASTEURELLA PSEUDOTUBERCULOSIS FROM ALASKA IDENTIFIED AS PASTEURELLA PESTIS: AN IMMUNOFLUORESCENT FALSE POSITIVE. Am J Trop Med Hyg. 1965 May;14:424–432. doi: 10.4269/ajtmh.1965.14.424. [DOI] [PubMed] [Google Scholar]
- WINTER C. C., CHERRY W. B., MOODY M. D. An unusual strain of Pasteurella pestis isolated from a fatal human case of plague. Bull World Health Organ. 1960;23:408–409. [PMC free article] [PubMed] [Google Scholar]
- WINTER C. C., MOODY M. D. Rapid identification of Pasteurella pestis with fluorescent antibody. II. Specific identification of Pasteurella pestis in dried smears. J Infect Dis. 1959 May-Jun;104(3):281–287. doi: 10.1093/infdis/104.3.281. [DOI] [PubMed] [Google Scholar]