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. 1968 May;16(5):691–694. doi: 10.1128/am.16.5.691-694.1968

An Improved Diluent for Rubella Hemagglutination and Hemagglutination-Inhibition Tests

Angela E Auletta 1,2, Gary L Gitnick 1,2, Carrie E Whitmire 1,2, John L Sever 1,2
PMCID: PMC547502  PMID: 5659363

Abstract

Rubella hemagglutinating (HA) antigen was prepared in BHK-21 tissue as 5% cell suspensions and from unconcentrated and 20× concentrated infected supernatant fluids. In some instances, unconcentrated fluids were treated with Tween 80 and ether; cell suspensions were treated with ether alone. Preparations were tested for HA activity in dextrose-gelatin-Veronal (DGV) buffer solutions; 0.85% NaCl; Sorenson's phosphate-buffered saline, pH 7.2; and a diluent of 0.9% NaCl, 0.1% CaCl2 (anhydrous), and 0.1% MgSO4·7H2O. HA titers were consistently two- to fourfold higher in the saline with added Ca++ and Mg++ than in DGV. Hemagglutination-inhibition titers of paired human sera were the same in either diluent. It is suggested that the interaction between rubella HA antigen and the red cells of young (less than 1-day-old) chicks may be at least partially ion dependent and that titers are enhanced by increased quantities of divalent cations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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