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. 1968 Jun;16(6):835–840. doi: 10.1128/am.16.6.835-840.1968

Stability of Suspensions of Influenza Virus Dried to Different Contents of Residual Moisture by Sublimation In Vacuo

Donald Greiff 1,2,3, Wilton A Rightsel 1,2,3
PMCID: PMC547538  PMID: 5664106

Abstract

After freezing, suspensions of influenza virus were dried by sublimation of water in vacuo to contents of residual moisture of 3.2, 2.1, 1.7, 1, or 0.4%. The stability of the several suspensions was determined by an accelerated storage test. Based on the times predicted for the dried preparations stored at different temperatures to lose 1 log of infectivity titer, the order of stability in relation to residual moistures was as follows: 1.7% > 2.1% > 1% > 3.2% > 0.4%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANASZAK L. J., GURD F. R. CARBOXYMETHYLATION OF SPERM WHALE METMYOGLOBIN. REACTIVITY OF THE ADJACENT HISTIDINE RESIDUES. J Biol Chem. 1964 Jun;239:1836–1838. [PubMed] [Google Scholar]
  2. FRY R. M., GREAVES R. I. N. The survival of bacteria during and after drying. J Hyg (Lond) 1951 Jun-Sep;49(2-3):220–246. doi: 10.1017/s0022172400044120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GREIFF D., RIGHTSEL W. A. AN ACCELERATED STORAGE TEST FOR PREDICTING THE STABILITY OF SUSPENSIONS OF MEASLES VIRUS DRIED BY SUBLIMATION IN VACUO. J Immunol. 1965 Mar;94:395–400. [PubMed] [Google Scholar]
  4. GREIFF D., RIGHTSEL W. A., SCHULER E. E. EFFECTS OF FREEZING, STORAGE AT LOW TEMPERATURES, AND DRYING BY SUBLIMATION IN VACUO ON THE ACTIVITIES OF MEASLES VIRUS. Nature. 1964 May 9;202:624–625. doi: 10.1038/202624a0. [DOI] [PubMed] [Google Scholar]
  5. Greiff D., Rightsel W. A. Stabilities of suspensions of viruses after freezing or drying by vacuum sublimation and storage. Cryobiology. 1967 May-Jun;3(6):432–444. doi: 10.1016/s0011-2240(67)80153-6. [DOI] [PubMed] [Google Scholar]
  6. HILLEMAN M. R., BUESCHER E. L., SMADEL J. E. Preparation of dried antigen and antiserum for the agglutination-inhibition test for virus influenza. Public Health Rep. 1951 Sep 21;66(38):1195–1203. [PMC free article] [PubMed] [Google Scholar]
  7. HUTTON R. S., HILMOE R. J., ROBERTS J. L. Some physical factors that influence the survival of Brucella abortus during freeze-drying. J Bacteriol. 1951 Mar;61(3):309–319. doi: 10.1128/jb.61.3.309-319.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Horowitz S. B., Fenichel I. R. Diffusion and the transport of organic nonelectrolytes in cells. Ann N Y Acad Sci. 1965 Oct 13;125(2):572–594. doi: 10.1111/j.1749-6632.1965.tb45415.x. [DOI] [PubMed] [Google Scholar]
  9. KAUZMANN W. Some factors in the interpretation of protein denaturation. Adv Protein Chem. 1959;14:1–63. doi: 10.1016/s0065-3233(08)60608-7. [DOI] [PubMed] [Google Scholar]
  10. KENDREW J. C. Side-chain interactions in myoglobin. Brookhaven Symp Biol. 1962 Dec;15:216–228. [PubMed] [Google Scholar]
  11. Naylor H. B., Smith P. A. Factors Affecting the Viability of Serratia marcescens During Dehydration and Storage. J Bacteriol. 1946 Nov;52(5):565–573. doi: 10.1128/jb.52.5.565-573.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Onsager L., Runnels L. K. MECHANISM FOR SELF-DIFFUSION IN ICE. Proc Natl Acad Sci U S A. 1963 Aug;50(2):208–210. doi: 10.1073/pnas.50.2.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Warner D. T. A proposed water-protein interaction and its application to the structure of the tobacco mosaic virus particle. Ann N Y Acad Sci. 1965 Oct 13;125(2):605–624. doi: 10.1111/j.1749-6632.1965.tb45417.x. [DOI] [PubMed] [Google Scholar]

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