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. 1968 Jun;16(6):841–844. doi: 10.1128/am.16.6.841-844.1968

A Precise Method for Replicating Suspension Cultures of Mammalian Cells1

Francis J Weirether 1, Jerry S Walker 1, Ralph E Lincoln 1
PMCID: PMC547539  PMID: 5664107

Abstract

A simple, readily assembled shaker-culture system for the cultivation of mammalian cells is described. No specialized glassware and equipment were used in this system, which consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed to quantitate the effects of several variables, including medium ingredients, serial transfers, and freezing and storage on two variants of the L-cell line. This system is reproducible and precise and allows for growth of cells in suspension for extended periods of time. Large numbers of cells can be mass-produced. Many replicates can be run simultaneously to yield data for statistical analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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