Abstract
Liquid nitrogen storage was the most satisfactory of several methods tested for supplying standard Streptomyces viridoflavus inoculum for laboratory and pilot plant experimentation. Shake-flask cultures were subdivided into sterile cotton-plugged ampoules and stored in the gas space of a liquid nitrogen refrigerator. There were no detectable changes in viability or in candidin-producing capacity over a 12-month test period. The procedure also proved satisfactory with all other organisms tested.
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Selected References
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- BURNS M. E. CRYOBIOLOGY AS VIEWED BY THE MICROBIOLOGIST. Cryobiology. 1964 Sep-Oct;51:18–39. doi: 10.1016/0011-2240(64)90017-3. [DOI] [PubMed] [Google Scholar]
- Hwang S. W. Long-term preservation of fungus cultures with liquid nitrogen refrigeration. Appl Microbiol. 1966 Sep;14(5):784–788. doi: 10.1128/am.14.5.784-788.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARTIN S. M. CONSERVATION OF MICROORGANISMS. Annu Rev Microbiol. 1964;18:1–16. doi: 10.1146/annurev.mi.18.100164.000245. [DOI] [PubMed] [Google Scholar]
- SOKOLSKI W. T., STAPERT E. M., FERRER E. B. LIQUID NITROGEN FREEZING IN MICROBIOLOGICAL ASSAY SYSTEMS. I. PRESERVATION OF LACTOBACILLUS LEICHMANNII FOR DIRECT USE IN THE VITAMIN B12 ASSAY. Appl Microbiol. 1964 Jul;12:327–329. doi: 10.1128/am.12.4.327-329.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Savage G. M., Brook M. J. The Fragmentation of the Mycelium of Penicillium notatum and Penicillium chrysogenum by a High-Speed Blender and the Evaluation of Blended Seed. J Bacteriol. 1946 Sep;52(3):385–391. [PMC free article] [PubMed] [Google Scholar]