Abstract
Enterotoxin A production in milk was studied by use of variables of milk quality, initial numbers of enterotoxigenic staphylococci, incubation temperature, and time. In both raw and pasteurized milks having a low total viable count, enterotoxin was detected in minimal incubation times of 6 to 9 hr at 35 C, 9 to 12 hr at 30 C, 18 hr at 25 C, and 36 hr at 20 C, after inoculation with 106Staphylococcus aureus cells per ml. When similar milks were inoculated with 104S. aureus cells per ml, enterotoxin was detected in 12 hr at 35 C, 18 hr at 30 C, 24 to 36 hr at 25 C, and 48 to 96 hr at 20 C. In high-count raw milk, enterotoxin was detected only in samples inoculated with 106S. aureus cells per ml and incubated at 35 C. Generally, a concentration of 5 × 107S. aureus cells per ml of milk was reached before enterotoxin A was detected.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANDERSON P. H., STONE D. M. Staphylococcal food poisoning associated with spray-dried milk. J Hyg (Lond) 1955 Dec;53(4):387–397. doi: 10.1017/s0022172400000899. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ARMIJO R., HENDERSON D. A., TIMOTHEE R., ROBINSON H. B. Food poisoning outbreaks associated with spray-dried milk; an epidemiologic study. Am J Public Health Nations Health. 1957 Sep;47(9):1093–1100. doi: 10.2105/ajph.47.9.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DONNELLY C. B., BLACK L. A., LEWIS K. H. OCCURRENCE OF COAGULASE-POSITIVE STAPHYLOCOCCI IN CHEDDAR CHEESE. Appl Microbiol. 1964 Jul;12:311–315. doi: 10.1128/am.12.4.311-315.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- READ R. B., Jr, PRITCHARD W. L., BRADSHAW J., BLACK L. A. IN VITRO ASSAY OF STAPHYLOCOCCAL ENTEROTOXINS A AND B FROM MILK. J Dairy Sci. 1965 Apr;48:411–419. doi: 10.3168/jds.s0022-0302(65)88245-5. [DOI] [PubMed] [Google Scholar]