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. 1968 Jul;16(7):1019–1023. doi: 10.21236/ad0838753

Rapid, Sensitive Assay for Staphylococcal Enterotoxin and a Comparison of Serological Methods

Sidney J Silverman 1, Allen R Knott 1, Mary Howard 1
PMCID: PMC547581  PMID: 4969665

Abstract

Reversed passive hemagglutination was used to assay enterotoxin in culture filtrates and in food samples. With cells tanned and then sensitized with antitoxin globulin and preserved with either formaldehyde or pyruvic aldehyde, as little as 0.0007 μg of enterotoxin was detectable. The results of hemagglutination tests compared well with those obtained by quantitative precipitin tests or by immunodiffusion, but hemagglutination was 50 to 100 times more sensitive than the immunodiffusion technique. In addition, results of the hemagglutination test were available within a few hours, and neither elimination of interfering proteins from food extracts nor concentration of the sample, both of which are necessary for immunodiffusion, was required for this procedure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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