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. 1968 Jul;16(7):1036–1039. doi: 10.1128/am.16.7.1036-1039.1968

Microtiter Method for the Evaluation of Viable Cells in Bacterial Cultures1

Daniel Y C Fung 1, Allen A Kraft 1
PMCID: PMC547584  PMID: 4874461

Abstract

A microtiter technique was investigated as a means of evaluating viable cells in bacterial cultures. Parallel experiments were performed employing the conventional agar plate method along with the microtiter procedure. Statistical analyses showed that the correlation between the two methods was highly significant. With this new method, many samples were analyzed simultaneously, and readable results were obtained in 12 to 15 hr. Other advantages of this method were substantial savings of time, space, and materials. Also, the applicability of this method to estimates of mixed bacterial populations was demonstrated by studying the associative growth of two bacterial cultures.

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Selected References

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  1. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]

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