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. 1968 Jul;16(7):1044–1052. doi: 10.1128/am.16.7.1044-1052.1968

Factors Influencing Agnotobiotic Cultures of the Rumen Ciliate, Entodinium simplex

B D W Jarvis 1,1, R E Hungate 1
PMCID: PMC547586  PMID: 4969666

Abstract

The nutrition of Entodinium simplex was studied, with foliage of bluegrass (Poa pratense), perennial ryegrass (Lolium perenne), and grains of wheat (Triticum vulgare) as substrates in agnotobiotic cultures. Entodinium grew poorly when the substrates were autoclaved; better growth was obtained when the substrates were sterilized with ethylene oxide vapor. The concentration of ethylene oxide and the amount of moisture influenced the sterility and nutritional adequacy of the treated substrate. Autolysates and hydrolysates of mixed rumen protozoa stimulated growth. Protozoal extracts did not replace factors destroyed by autoclaving. Clarified rumen fluid assisted the cultivation of entodinia from small inocula but was detrimental to established cultures. Success of cultures was influenced by the medium used to grow the inoculum as well as by the medium inoculated. The results indicated that the composition of the bacterial population influences the growth of E. simplex.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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