Abstract
Cultures of Bordetella pertussis cultivated in shake flasks were invariably highly toxic for mice, but cultures of the same strain grown in vortex-aerated vessels were nontoxic at the time of harvest. Results reported here indicate that toxin is present during the early log phase in vortex-aerated cultures, but is lost as the cultivation proceeds. The loss of toxicity is apparently due to denaturation of the toxin by the combined influence of vigorous aeration and elevated pH.
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Selected References
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- BANERJEA A., MUNOZ J. Antigens of Bordetella pertussis. II. Purification of heat-labile toxin. J Bacteriol. 1962 Aug;84:269–274. doi: 10.1128/jb.84.2.269-274.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen S. M., Wheeler M. W. Pertussis Vaccine Prepared with Phase-I Cultures Grown in Fluid Medium. Am J Public Health Nations Health. 1946 Apr;36(4):371–376. [PMC free article] [PubMed] [Google Scholar]
- ONOUE K., KITAGAWA M., YAMAMURA Y. CHEMICAL STUDIES ON CELLULAR COMPONENTS OF BORDETELLA PERTUSSIS. III. ISOLATION OF HIGHLY POTENT TOXIN FROM BORDETELLA PERTUSSIS. J Bacteriol. 1963 Oct;86:648–655. doi: 10.1128/jb.86.4.648-655.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PENNELL R. B., THIELE E. H. Studies on the fractionation of Hemophilus pertussis extracts. J Immunol. 1951 Jun;66(6):627–633. [PubMed] [Google Scholar]
- PITTMAN M., COX C. B. PERTUSSIS VACCINE TESTING FOR FREEDOM-FROM-TOXICITY. Appl Microbiol. 1965 May;13:447–456. doi: 10.1128/am.13.3.447-456.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PITTMAN M. Influence of preservatives of heat, and of irradiation on mouse protective activity and detoxification of pertussis vaccine. J Immunol. 1952 Aug;69(2):201–216. [PubMed] [Google Scholar]