Abstract
Acid production in milk by Lactobacillus bulgaricus was stimulated by tomato juice or its serum. Preliminary purification of the stimulants involved adsorption and elution on a cation-exchange resin. Unidimensional paper chromatography in two solvent systems was employed in further isolation and purification. Identification of the stimulatory components was based on ultraviolet spectral analysis and thin-layer chromatography. The stimulants were identified as adenine and adenosine.
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Selected References
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- AURAND L. W., HEIMBUCH A. H., SPECK M. L. Some characteristics of a growth stimulant in corn steep liquor for Lactobacillus casei. J Bacteriol. 1956 Oct;72(4):543–547. doi: 10.1128/jb.72.4.543-547.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- IKAWA M., O'BARR J. S. The nature of some growth stimulatory substances for Lactobacillus delbrueckii. J Bacteriol. 1956 Apr;71(4):401–405. doi: 10.1128/jb.71.4.401-405.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KORNBERG A., PRICER W. E., Jr Enzymatic phosphorylation of adenosine and 2,6-diaminopurine riboside. J Biol Chem. 1951 Dec;193(2):481–495. [PubMed] [Google Scholar]
- LAMPEN J. O., WANG T. P. The mechanism of action of Lactobacillus pentosus nucleosidase. J Biol Chem. 1952 Sep;198(1):385–395. [PubMed] [Google Scholar]
- MCANELLY J. K., SPECK M. L. Amino acid content of a peptide stimulatory for Lactobacillus casei. J Bacteriol. 1957 May;73(5):676–681. doi: 10.1128/jb.73.5.676-681.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Metcalf D., Hucker G. J., Carpenter D. C. A Growth Factor in Certain Vegetable Juices. J Bacteriol. 1946 Mar;51(3):381–384. [PMC free article] [PubMed] [Google Scholar]
- STAMER J. R., ALBURY M. N., PEDERSON C. S. SUBSTITUTION OF MANGANESE FOR TOMATO JUICE IN THE CULTIVATION OF LACTIC ACID BACTERIA. Appl Microbiol. 1964 Mar;12:165–168. doi: 10.1128/am.12.2.165-168.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snell E. E., Mitchell H. K. Purine and Pyrimidine as Growth Substances for Lactic Acid Bacteria. Proc Natl Acad Sci U S A. 1941 Jan 15;27(1):1–7. doi: 10.1073/pnas.27.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZURAW E. A., SPECK M. L., AURAND L. W., TOVE S. B. Purification of stimulants from condensed corn-fermentation solubles active for Lactobacillus casei in milk. J Bacteriol. 1960 Oct;80:457–463. doi: 10.1128/jb.80.4.457-463.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
