Abstract
A culture enrichment technique was used to isolate phytase-producing microorganisms. Also, microorganisms from various culture collections were tested for their phytase-producing ability. A number of the Aspergillus niger group produced extracellular phytase which dephosphorylated calcium phytate in acidic solution. A soil isolate, A. ficuum NRRL 3135, produced the most active phytase in a cornstarch-based medium. Production of phytase was strongly repressed by inorganic phosphates and required a high carbon to phosphorus ratio in the medium.
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- Greaves M. P., Anderson G., Webley D. M. The hydrolysis of inositol phosphates by Aerobacter aerogenes. Biochim Biophys Acta. 1967 Mar 15;132(2):412–418. doi: 10.1016/0005-2744(67)90160-x. [DOI] [PubMed] [Google Scholar]
- MAYER A. M. The breakdown of phytin and phytase activity in germinating lettuce seeds. Enzymologia. 1958 Jan 31;19(1):1–8. [PubMed] [Google Scholar]
- MAYER F. C., CAMPBELL R. E., SMITH A. K., MCKINNEY L. L. Soybean phosphatase. Purification and properties. Arch Biochem Biophys. 1961 Aug;94:301–307. doi: 10.1016/0003-9861(61)90044-3. [DOI] [PubMed] [Google Scholar]
- Nelson T. S. The utilization of phytate phosphorus by poultry--a review. Poult Sci. 1967 Jul;46(4):862–871. doi: 10.3382/ps.0460862. [DOI] [PubMed] [Google Scholar]
- PEERS F. G. The phytase of wheat. Biochem J. 1953 Jan;53(1):102–110. doi: 10.1042/bj0530102. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw J. G. Acid phosphatase from tobacco leaves. Arch Biochem Biophys. 1966 Oct;117(1):1–9. doi: 10.1016/0003-9861(66)90118-4. [DOI] [PubMed] [Google Scholar]
- TAYLOR T. G. THE AVAILABILITY OF THE CALCIUM AND PHOSPHORUS OF PLANT MATERIALS FOR ANIMALS. Proc Nutr Soc. 1965;24:105–112. doi: 10.1079/pns19650017. [DOI] [PubMed] [Google Scholar]