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. 1968 Sep;16(9):1406–1413. doi: 10.1128/am.16.9.1406-1413.1968

Cellular Fatty Acid Composition and Identification of Rumen Bacteria1

R W Ifkovits 1,2, H S Ragheb 1
PMCID: PMC547664  PMID: 5692111

Abstract

The fatty acid compositions of 21 pure cultures of rumen bacteria, representing 12 genera and 14 species, were compared as methyl esters. Each organism possessed a consistent and reproducible fatty acid profile. Overlapping similarities and differences in composition did not allow differentiation between families or genera. Although species differentiation was possible, fatty acid composition appeared to be only an aid in the identification of bacteria.

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Selected References

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  1. ABEL K., DESCHMERTZING H., PETERSON J. I. CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION. I. FEASIBILITY OF UTILIZING GAS CHROMATOGRAPHY. J Bacteriol. 1963 May;85:1039–1044. doi: 10.1128/jb.85.5.1039-1044.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ALLISON M. J., BRYANT M. P., KATZ I., KEENEY M. Metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. II. Biosynthesis of higher branched-chain fatty acids and aldehydes. J Bacteriol. 1962 May;83:1084–1093. doi: 10.1128/jb.83.5.1084-1093.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BRAGG P. D., REEVES R. E. Studies on the carbohydrate metabolism of a gram-negative anaerobe (Bacteroides symbiosus) used in the culture of Entamoeba histolytica. J Bacteriol. 1962 Jan;83:76–84. doi: 10.1128/jb.83.1.76-84.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BRYANT M. P. Bacterial species of the rumen. Bacteriol Rev. 1959 Sep;23(3):125–153. doi: 10.1128/br.23.3.125-153.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BRYANT M. P., SMALL N. The anaerobic monotrichous butyric acid-producing curved rod-shaped bacteria of the rumen. J Bacteriol. 1956 Jul;72(1):16–21. doi: 10.1128/jb.72.1.16-21.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ikawa M. Bacterial phosphatides and natural relationships. Bacteriol Rev. 1967 Mar;31(1):54–64. doi: 10.1128/br.31.1.54-64.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kanegasaki S., Takahashi H. Function of growth factors for rumen microorganisms. II. Metabolic fate of incorporated fatty acids in Selenomonas ruminantium. Biochim Biophys Acta. 1968 Jan 10;152(1):40–49. [PubMed] [Google Scholar]
  8. Kates M. Bacterial lipids. Adv Lipid Res. 1964;2:17–90. [PubMed] [Google Scholar]
  9. Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moss C. W., Lewis V. J. Characterization of clostridia by gas chromatography. I. Differentiation of species by cellular fatty acids. Appl Microbiol. 1967 Mar;15(2):390–397. doi: 10.1128/am.15.2.390-397.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reiner E. Identification of bacterial strains by pyrolysis-gas-liquid chromatography. Nature. 1965 Jun 19;206(990):1272–1273. doi: 10.1038/2061272b0. [DOI] [PubMed] [Google Scholar]
  12. Steinhauer J. E., Flentge R. L., Lechowich R. V. Lipid patterns of selected microorganisms as determined by gas-liquid chromatography. Appl Microbiol. 1967 Jul;15(4):826–829. doi: 10.1128/am.15.4.826-829.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WARNER A. C. Enumeration of rumen micro-organisms. J Gen Microbiol. 1962 Apr;28:119–128. doi: 10.1099/00221287-28-1-119. [DOI] [PubMed] [Google Scholar]
  14. WEGNER G. H., FOSTER E. M. Incorporation of isobutyrate and valerate into cellular plasmalogen by Bacteroides succinogenes. J Bacteriol. 1963 Jan;85:53–61. doi: 10.1128/jb.85.1.53-61.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. YAMAKAWA T., UETA N. GASCHROMATOGRAPHIC STUDIES OF MICROBIAL COMPONENTS. I. CARBOHYDRATE AND FATTY ACID CONSTITUTION OF NEISSERIA. Jpn J Exp Med. 1964 Dec;34:361–374. [PubMed] [Google Scholar]

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