Abstract
Methods and procedures are described for the identification of aerobic pseudomonads isolated from clinical specimens. Fluorescence is used as a means of primary differentiation. Final identification is based on the observation of characteristics such as flagellation, storage of intracellular fat, growth at 4 C and at 41 C, denitrification, gelatin hydrolysis, arginine dihydrolase activity, and oxidase activity. The value of this schema to the diagnostic laboratory is discussed.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burdon K. L. Fatty Material in Bacteria and Fungi Revealed by Staining Dried, Fixed Slide Preparations. J Bacteriol. 1946 Dec;52(6):665–678. doi: 10.1128/jb.52.6.665-678.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GABY W. L., FREE E. Occurrence and identification of nonpigmented strains of Pseudomonas aeruginosa in the clinical laboratory. J Bacteriol. 1953 Jun;65(6):746–746. doi: 10.1128/jb.65.6.746-746.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAYNES W. C. Pseudomonas aeruginosa--its characterization and identification. J Gen Microbiol. 1951 Nov;5(5 Suppl):939–950. doi: 10.1099/00221287-5-5-939. [DOI] [PubMed] [Google Scholar]
- HUGH R., RYSCHENKOW E. Pseudomonas maltophilia, an alcaligenes-like species. J Gen Microbiol. 1961 Sep;26:123–132. doi: 10.1099/00221287-26-1-123. [DOI] [PubMed] [Google Scholar]
- KOVACS N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature. 1956 Sep 29;178(4535):703–703. doi: 10.1038/178703a0. [DOI] [PubMed] [Google Scholar]
- LEIFSON E. Identification of Pseudomonas, Alcaligenes and related bacteria. Zentralbl Bakteriol Orig. 1958 Dec;173(5-6):487–488. [PubMed] [Google Scholar]
- LEIFSON E. Staining, shape and arrangement of bacterial flagella. J Bacteriol. 1951 Oct;62(4):377–389. doi: 10.1128/jb.62.4.377-389.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leifson E. Bacterial taxonomy: a critique. Bacteriol Rev. 1966 Jun;30(2):257–266. doi: 10.1128/br.30.2.257-266.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MOORE H. B., PICKETT M. J. The Pseudomonas-Achromobacter group. Can J Microbiol. 1960 Feb;6:35–42. doi: 10.1139/m60-006. [DOI] [PubMed] [Google Scholar]
- Redfearn M. S., Palleroni N. J., Stanier R. Y. A comparative study of Pseudomonas pseudomallei and Bacillus mallei. J Gen Microbiol. 1966 May;43(2):293–313. doi: 10.1099/00221287-43-2-293. [DOI] [PubMed] [Google Scholar]
- SHERRIS J. C., SHOESMITH J. G., PARKER M. T., BRECKON D. Tests for the rapid breakdown of arginine by bacteria: their use in the identification of pseudomonads. J Gen Microbiol. 1959 Oct;21:389–396. doi: 10.1099/00221287-21-2-389. [DOI] [PubMed] [Google Scholar]
- Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
- Sutter V. L., Hurst V., Landucci A. O. Pseudomonads in human saliva. J Dent Res. 1966 Nov-Dec;45(6):1800–1803. doi: 10.1177/00220345660450063601. [DOI] [PubMed] [Google Scholar]
- Sutter V. L., Hurst V., Lane C. W. Quantification of Pseudomonas aeruginosa in feces of healthy human adults. Health Lab Sci. 1967 Oct;4(4):245–249. [PubMed] [Google Scholar]
- VONGRAEVENITZ A. PSEUDOMONAS STUTZERI ISOLATED FROM CLINICAL SPECIMENS. Am J Clin Pathol. 1965 Apr;43:357–360. [PubMed] [Google Scholar]
- WAHBA A. H., DARRELL J. H. THE IDENTIFICATION OF ATYPICAL STRAINS OF PSEUDOMONAS AERUGINOSA. J Gen Microbiol. 1965 Mar;38:329–342. doi: 10.1099/00221287-38-3-329. [DOI] [PubMed] [Google Scholar]