Erratum to: Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system

Erratum to: Biotechnol Biofuels (2016) 9:39 DOI 10.1186/s13068-016-0452-y

After the publication of the article [1], it was brought to our attention that some of the data in Table 2 were incorrect. Please find a correct and updated version of Table 2 in the erratum. Following this Fig. 1 has also been updated; the correct version of Fig. 1 is given in this erratum.

Table 2.

Key process parameters of anaerobic glucose conversion of P. putida F1 in the anode compartment of a BES using [Co(bipy)₃]^3+/2+ or [Fe(CN)₆]^3−/4− as electron acceptors while poising the anode at +0.697 V vs SHE

	[Co(bipy)3]3+/2+	[Fe(CN)6]3−/4−
Carbon balance (%)	99.6	97.6
Coulombic efficiency (%)	98.5	93.3
Yields (molproduct/molglucose)
Y 2KGA	0.90 ± 0.03	0.90 ± 0.02
Y acetic acid	0.073 ± 0.008	0.144 ± 0.012
Y gluconic acid	0.31 ± 0.06	0.09 ± 0.03
	0.25 ± 0.03	0.09 ± 0.04
Y electrons	3.94 ± 0.11	3.88 ± 0.07
Rates (mmol/(gCDW h))
r glucose	−0.26 ± 0.04	−0.35 ± 0.07
r acetic acid	0.019 ± 0.003	0.051 ± 0.010
r 2KGA	0.23 ± 0.04	0.32 ± 0.06
r gluconic acid	0.08 ± 0.02	0.03 ± 0.01
	−0.06 ± 0.01	−0.03 ± 0.02
r electrons	1.02 ± 0.18	1.37 ± 0.26

Data are fitted with linear regression using datasets from ten ([Fe(CN)₆]^3−/4−) and four ([Co(bipy)₃]^3+/2+) biological replicates with a total of 79 and 36 samples, respectively (compare Additional file 1: Fig. S3). Carbon balance is calculated from the fitted rates considering carbon content of molecules and assuming equimolar CO₂ production when making acetate from glucose. Gluconic acid is a product in the first 100 h and serves as a substrate thereafter, hence 2 yields and rates are given

Fig. 1.

Change of biomass (triangles, a), pH (squares, b) and electron production (circles, b) in the anode compartment of a BES reactor of P. putida F1 with K₃[Fe(CN)₆] as electron acceptor in control (black symbols) and closed circuit with the anode potential poised at +0.697 V (white symbols). Data have been averaged from ten (closed circuit) and three (control) biological replicates with a total of 79 and 30 samples, respectively. Means and standard deviations (X and Y error bars) are given [average sample size n = 7 (closed circuit); exact sample size n = 3 (control)]

Also, during the calculation of specific glucose uptake rate, the authors mistakenly used the unit mmol/L as mmol, and therefore it caused some errors in the calculations of production rate (Table 2) and ATP regeneration rate (Section “Flux balance analysis”—[1]) which need to be corrected. The corrected ATP regeneration rates are 0.02 and 0.38 mmol_ATP/(gCDW h) for [Co(bpy)₃](ClO₄)₂ from glucose oxidation and membrane-bound ATP synthase respectively, while those numbers for K₃[Fe(CN)₆] are 0.05 and 0.64 mmol_ATP/(gCDW h), respectively.