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. 1968 Nov;16(11):1695–1698. doi: 10.1128/am.16.11.1695-1698.1968

Detection of Salmonella by a Single-Culture Technique

Kerstin Abrahamsson 1, G Patterson 1,1, H Riemann 1
PMCID: PMC547741  PMID: 4881954

Abstract

Dulcitol-selenite enrichment medium in a motility flask was used for the detection of Salmonella in food. A drop in pH of the dulcitol-selenite enrichment motility broth indicated the presence of Salmonella; this phenomenon was confirmed by fluorescent-antibody staining. A complete correlation was found between fluorescent-antibody staining and recovery on Brilliant Green agar. Testing of 332 samples of 8 different kinds of foods and feeds indicated no significant difference in sensitivity between the new technique and a conventional Salmonella detection technique. The new technique permitted detection of even small numbers of Salmonella in 1 to 2 days.

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Selected References

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  1. Caldwell W. J., Stulberg C. S., Peterson W. D., Jr Somatic and flagellar immunofluorescence of Salmonella. J Bacteriol. 1966 Oct;92(4):1177–1187. doi: 10.1128/jb.92.4.1177-1187.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. GALTON M. M., SCATTERDAY J. E., HARDY A. V. Salmonellosis in dogs. I. Bacteriological, epidemiological and clinical considerations. J Infect Dis. 1952 Jul-Aug;91(1):1–5. doi: 10.1093/infdis/91.1.1. [DOI] [PubMed] [Google Scholar]
  3. GEORGALA D. L., BOOTHROYD M. A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT. J Hyg (Lond) 1964 Sep;62:319–327. doi: 10.1017/s0022172400040031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Georgala D. L., Boothroyd M., Hayes P. R. Further evaluation of a rapid immunofluorescence technique for detecting salmonellae in meat and poultry. J Appl Bacteriol. 1965 Dec;28(3):421–425. doi: 10.1111/j.1365-2672.1965.tb02172.x. [DOI] [PubMed] [Google Scholar]
  5. HAGLUND J. R., AYRES J. C., PATON A. M., KRAFT A. A., QUINN L. Y. DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY. Appl Microbiol. 1964 Sep;12:447–450. doi: 10.1128/am.12.5.447-450.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harvey R. W., Mahabir D. E., Price T. H. A method of secondary enrichment for salmonellas independent of selectively toxic chemicals. J Hyg (Lond) 1966 Sep;64(3):361–366. doi: 10.1017/s002217240004064x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. INO J., GRABER C. D. Recovery of Salmonella from contaminated cultures. U S Armed Forces Med J. 1955 Apr;6(4):586–587. [PubMed] [Google Scholar]
  8. Insalata N. F., Schulte S. J., Berman J. H. Immunofluorescence technique for the detection of salmonellae in various foods. Appl Microbiol. 1967 Sep;15(5):1145–1149. doi: 10.1128/am.15.5.1145-1149.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KONFORTI N., NAVON B., RAPPAPORT F. A new enrichment medium for certain Salmonellae. J Clin Pathol. 1956 Aug;9(3):261–266. doi: 10.1136/jcp.9.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PIJPER A. Dimensional differentiation, filtration and separation of bacteria. J Pathol Bacteriol. 1952 Jul;64(3):529–538. doi: 10.1002/path.1700640314. [DOI] [PubMed] [Google Scholar]
  11. Raj H. Enrichment medium for selection of Salmonella from fish homogenate. Appl Microbiol. 1966 Jan;14(1):12–20. doi: 10.1128/am.14.1.12-20.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STUART P. F., PIVNICK H. ISOLATION OF SALMONELLAE BY SELECTIVE MOTILITY SYSTEMS. Appl Microbiol. 1965 May;13:365–372. doi: 10.1128/am.13.3.365-372.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. THOMASON B. M., CHERRY W. B., EDWARDS P. R. Staining bacterial smears with fluorescent antibody. VI. Identification of Salmonellae in fecal specimens. J Bacteriol. 1959 Apr;77(4):478–486. doi: 10.1128/jb.77.4.478-486.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. THOMASON B. M., CHERRY W. B., MOODY M. D. Staining bacterial smears with fluorescent antibody. III. Antigenic analysis of Salmonella typhosa by means of fluorescent antibody and agglutination reactions. J Bacteriol. 1957 Oct;74(4):525–532. doi: 10.1128/jb.74.4.525-532.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

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