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. 2017 Apr 12;20:79–97. doi: 10.1016/j.ebiom.2017.04.015

Fig. 3.

Fig. 3

Knockdown of RIOK1 in RAS-transformed colon cancer cell lines inhibits proliferation and metabolism. (a) Representative RIOK1 knockdown Western blot analysis for SW480 cells (N = 3). (b) Colony formation assays of SW480 cells. Formed colonies were stained after 7 days and cell density was determined. Representative pictures are shown. Data is represented as mean ± SEM with ** for p < 0.01 by ordinary two-way ANOVA (Tukey) compared to non-sil. dox. N = 3. (c) SW480 cells were seeded in 2% Matrigel and grown for 13 days. Scale bar: 200 μm. (d) MTT assay measured on day 3 for SW480 cells. Data is represented as mean ± SEM; N = 2. (e) RIOK1 knockdown Western blot analysis for HCT116 cells (N = 1). (f) Colony formation assays of HCT116 cells. Formed colonies were stained after 10 days and cell density was determined. Representative pictures are shown. Data is represented as mean ± SEM with * for p < 0.05 by ordinary two-way ANOVA (Dunnett) compared to non-sil. dox; N = 4. (g) HCT116 cells were seeded in 2% Matrigel and grown for 18 days. Scale bars: 200 μm. (h) MTT assay measured on indicated timepoints and normalized to day 0 for HCT116 cells. Data is represented as mean ± SEM with * for p < 0.05 by two-way ANOVA (Tukey); N = 3.