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. 1990 Oct;87(19):7551–7554. doi: 10.1073/pnas.87.19.7551

Antibody caging of a nuclear-targeting signal.

M S Halleck 1, M Rechsteiner 1
PMCID: PMC54785  PMID: 2170983

Abstract

We have developed a technique for reversibly masking a peptide-targeting signal. A fluoresceinated derivative of the simian virus 40 large tumor antigen nuclear-targeting signal was synthesized and cross-linked to bovine serum albumin. The conjugated protein was efficiently transported into rat liver nuclei unless the peptide-targeting signal was sterically hindered by binding of an anti-fluorescein antibody. Addition of free 5-aminofluorescein competed for antibody binding and rapidly restored nuclear accumulation of the derivatized bovine serum albumin. General use of hapten derivatization and anti-hapten antibodies for caging portions of macromolecular surfaces can be extended to a variety of proteins, including antibodies themselves.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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