FIG 3 .

Involvement of nuo genes in CafA-mediated coaggregation and pilus assembly. (A) The nuo operon and adjacent genes are shown in black, and genes involved in ubiquinone/menaquinone biosynthesis are shown in gray. Arrowheads indicate the locations of the Tn5 transposon. (B) Coaggregation of A. oris strains and S. oralis So34 was performed as described in the legend to Fig. 2. (C to J) A. oris cells were immobilized in nickel-carbon grids and labeled with anti-CafA antibodies, followed by labeling with anti-rabbit IgG antibodies conjugated to 18-nm gold particles. Samples were stained with 1% uranyl acetate and viewed by a transmission electron microscope. Scale bars indicate 0.5 µm.