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. 2017 Jun 16;83(13):e00987-17. doi: 10.1128/AEM.00987-17

Correction for Wertz et al., “Genomic and Physiological Characterization of the Verrucomicrobia Isolate Didymococcus colitermitum gen. nov., sp. nov., Reveals Microaerophily and Nitrogen Fixation Genes”

John T Wertz a, Eunji Kim a, John A Breznak b, Thomas M Schmidt b, Jorge L M Rodrigues c
PMCID: PMC5479004  PMID: 28625969

AUTHOR CORRECTION

Volume 78, no. 5, p. 1544–1555, 2012, https://doi.org/10.1128/AEM.06466-11. The name Diplosphaera, the proposed genus name for Verrucomicrobia strain TAV2, was previously used for a microalga. The revised genus and species name for the organism in our paper are described below.

Page 1544: The title should read as shown above.

Page 1544, abstract, lines 13 and 14: “Diplosphaera colitermitum” should read “Didymococcus colitermitum.”

Pages 1545, 1547, 1549, 1551, 1553, and 1555, running title: “Diplosphaera colitermitum” should read “Didymococcus colitermitum.”

Page 1549, Table 1, caption: “Diplosphaera colitermitum” should read “Didymococcus colitermitum.”

Page 1550, Table 2, caption: “Diplosphaera colitermitum” should read “Didymococcus colitermitum.”

Page 1553, column 1, paragraph 2: The heading for this section should be “Proposal for a new taxon, Didymococcus colitermitum gen. nov., sp. nov.” and “Diplosphaera colitermitum” should read “Didymococcus colitermitum.”

Page 1553, column 1: Paragraphs 3 and 4 should read as follows:

Description of Didymococcus gen. nov. Didymococcus gen. nov. (Di.dy.mo.coc'cus. Gr. fem. n. didymos pair; N.L. masc. n. coccus (from Gr. masc. n. kokkos, grain, seed), coccus; N.L. masc. n. Didymococcus a coccus in pairs).

Description of Didymococcus colitermitum sp. nov. Didymococcus colitermitum sp. nov. (co.li.ter'mi.tum. L. n. colon, colon, part of the large intestine; L. n. termes -itis, wood-eating worm, termite; N.L. gen. pl. n. colitermitum, of the gut of termites). Cells are coccoid (0.25 μm to 0.5 μm in diameter) and occur almost exclusively in pairs, with a Gram-negative cell wall morphology that includes an outer membrane. Cells are nonmotile, obligate aerobes and are microaerophilic. The shortest generation times occur in liquid medium under an atmosphere of 2 to 8% O2 (balance N2). On solid R2A medium, colonies are 2 to 4 mm in diameter, have an entire margin and a low convex, mucoid morphology, and are cream colored. Cells do not possess catalase or NADH/NADPH peroxidase activity. Nitrogenase activity is inferred through growth on nitrogen-free medium. Growth occurs in liquid media between 15 and 35°C (optimum, 30°C); there is no growth at 37°C or 4°C. Growth occurs at a pH range of 5.5 to 7.5 (optimum, 7.0); there is no growth at a pH of ≤5 or ≥8. Substrates utilized as energy sources include starch, d-cellobiose, d-maltose, d-glucose, d-galactose, and one or more components present in yeast extract. Microcrystalline cellulose, methylcellulose, carboxy methylcellulose, xylan, d-fructose, d-mannose, d-trehalose, sucrose, d-ribose, d-xylose, l-arabinose, d-mannitol, d-sorbitol, d-raffinose, dl-lactate, sodium pyruvate, sodium fumarate, sodium acetate, allantoin, d-glucuronate, d-galacturonate, d-gluconic acid, xanthine, tannic acid, resourcinol, vanillic acid, sodium benzoate, and trimethylbenzoate are not utilized. The genome of type strain TAV2 is 5.2 Mb in size, contains 60.5 mol% G+C, and possesses one 16S rRNA gene copy. The type strain, isolated from guts of Reticulitermes flavipes (Kollar) collected in Dansville, MI, is TAV2 (ATCC BAA-2264; DSM 25453).


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