Fig. 4.

vpr-1 acts primarily in the germ line and nervous system to promote gonadogenesis. (A) Genetic mosaic analysis showing cell lineages of major tissues. Each circle or square indicates one genetic mosaic worm. Points at which the genomic copy of vpr-1(+) was lost and the resulting phenotype are indicated. (B) Representative DIC images of gonads in vpr-1(tm1411) mosaic worms. Ex vpr-1(+) indicates expression of the vpr-1 genomic locus via an extrachromosomal array. Gonads are outlined in white. Arrowhead indicates an endomitotic oocyte that failed to ovulate and the arrow indicates a proximal germ cell tumor (Pro phenotype). Both phenotypes can arise from abnormal somatic gonad development or function (Hubbard and Greenstein, 2000; Killian and Hubbard, 2005).