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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Virology. 2017 Feb 10;504:122–140. doi: 10.1016/j.virol.2017.01.023

Figure 1. GPCMV major immediate early (MIE) locus encodes IE1 and IE2.

Figure 1

(i) Schematic representation of the GPCMV BAC genome and major immediate-early region with HindIII sites indicated. (ii) The GPCMV MIE WT locus is located within the HindIII ‘B’ fragment and encodes exons 1 to 5 on the antisense strand under the control of the MIE promoter (GPCMV MIEp) (purple). (iii) GPCMV IE1 and IE2 are splice variants that share exons 2 and 3, but are distinct in unique exon 4 (IE1, GP123) and 5 (IE2, GP122). Exon 1 is not translated. (iv) GP123Km (A–B) and GP122Km (C–D) mutant BACs were separately transfected onto GPL cells to generate virus. Each BAC was transfected individually (A and C) or in combination with a rescue plasmid encoding a WT IE1 or IE2 locus (B and D). Images taken 20 days post transfection.