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105 mouse spermatozoa stained with Hoechst 33342 (green) were incubated in the absence (A) or presence (B) of 50 µg/ml of semen fibrils at 37°C in a final volume of 100 µl for 15–20 min. Images were acquired for 20 s with an interval of 1 s on an LSM710 confocal microscope (Zeiss) using a 20X air objective.
