Apoptosis-inducing effect of myxoma virus on human neuroglioma cell lines

Abstract

The purpose of this study was to further evaluate the role of myxoma virus (MYXV) as an oncolytic agent against experimental human gliomas in vitro, and analyze the effect of MYXV on malignant glioma cells at different incubation periods and infected at different multiplicities of infection. Neuroglioma cell lines U251 and A172 were cultured with various infective doses of myxoma virus at different time points (0–3 days) and cellular survival rates were evaluated using an MTT assay. Cell viability and cell death rates were assessed using Annexin V/propidium iodide and applying flow cytometry. Furthermore, the expression levels of phosphorylated AKT (p-AKT) in malignant gliomas were detected by western blot analysis to investigate the possible cell signaling targets in the pathway. MYXV exhibited a dose and time-dependent cytotoxic effect on neuroglioma cells, and there was increased expression of p-AKT in malignant gliomas. The present study confirms that MYXV induces oncolysis of malignant gliomas through regulating the activation of AKT. As such, MYXV is a potential therapeutic agent against human malignant gliomas.

Introduction

Gliomas occur in ectodermal tissue and are characterized by aggressive proliferation and expansion into surrounding brain tissue. They reportedly account for ~50% of the incidence rate of neuroepithelial tumors (1). Malignant gliomas are invasive and rapidly growing tumors, which are refractory to available treatments, so they continue to be a major therapeutic challenge. Long-term survivors are rare and the median survival of patients with malignant gliomas is ~1 year, which has not changed notably in the last 30 years (2). In recent years, glioma therapy has advanced substantially, especially due to molecular biology research. Gene and viral therapies are gradually being developed, with several previous studies and a small number of pre-clinical research reports focused on this area (3–5).

Myxoma virus (MYXV) is a type of poxvirus and has a large double-stranded DNA genome that allows for the potential insertion of large (25 kb), therapeutically relevant, eukaryotic genes (6). MYXV is a rabbit-specific virus and brings about a lethal disease in the European rabbit (7,8). However, it is an oncolytic virus that is non-pathogenic for all other vertebrate species tested including humans. Despite the extremely narrow species host range, MYXV can effectively infect certain non-rabbit cells in vitro, including primary murine cells with genetic defects of the interferon response, and a variety of human tumor cells in vitro experiments (9–12). It has been shown in previous studies that MYXV exerts an effective and oncolytic potential against human malignant gliomas (8,9). The mechanism underlying the progress of this oncolysis is not yet fully understood. However, there is increasing evidence that the activity of AKT plays a central role in modulating MYXV-mediated oncolysis of diverse tumor cells (13,14).

In this study, MYXV was shown to affect cell viability and induce cell apoptosis, resulting in a dose- and time-dependent cytotoxic effect on neuroglioma cells. The phosphorylation of AKT in the neuroglioma cell lines was induced by MYXV, indicating that MYXV induced oncolysis of malignant gliomas through regulating the activation of AKT. These results suggest that MYXV could have therapeutic value in the treatment of malignant gliomas.

Materials and methods

Cell cultures

The human neuroglioma cells lines U251 (TCHu 58) and A172 (TCHu171) obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and were grown in DMEM/F12 (Hyclone, Beijing, China) containing 10% fetal bovine serum (FBS; Hyclone) at 37°C in a humidified 5% CO2 incubator. All cells were passaged until they reached 80% confluence, harvested by trypsin treatment, and replaced in the medium. Each cell line was tested routinely for mycoplasma contamination.

Virus and cell infection

Parental myxoma virus (Lausanne strain) was obtained from Grant McFadden at the University of Florida (Gainsville, FL, USA) and was amplified in BGMK cells as previously described (15). In brief, BGMK cells were infected with myxoma virus and then harvested following 72 h. These cells were lysed through filtration, and then the supernatant was clarified via a 36% sucrose pad. Pellets in the supernatant were eliminated by discontinuous (40/36/32/28/24) percent sucrose gradient and viral virions were extracted from the 40/36% interface. A derivative of myxoma virus, designated vMyxGFP (16), was prepared and titrated on BGMK cells as described previously (15). Inactive myxoma virus was prepared by irradiating vMyxGFP with UV light for 3 h. U251 and A172 cells were infected with vMyxGFP or inactive myxoma virus at multiplicities of infection (MOIs) of 0, 0.1, 0.5, 1, 5 and 10 for 1 h at 37°C, and then the cells were washed with phosphate-buffered saline (PBS) and cultured with fresh medium for subsequent experiments.

MTT assay

MTT assay was used to detect the viability of cells infected with 6 gradients (0, 0.1, 0.5, 1, 5 and 10) of multiplicity of infection (MOI) of MYXV in U251 and A172 cells. The MOI 5, with 40–60% cell lethality was then used in the subsequent experiments. In brief, cells were plated by using fresh DMEM/F12 medium (Hyclone) supplemented with 10% FBS in 96-well plates at a density of 5,000 cells per well. Following overnight cultivation at 37°C, cells were infected with MYXV for 1, 2 or 3 days. After incubating with an additional 25 µl MTT solution (5 mg/ml) for 4 h under 5% CO2 at 37°C, 100 µl dimethyl sulfoxide (DMSO) was added and the container was agitated for 5 min. The precipitated formazan was dissolved, and detected using a microplate reader at 595 nm.

Cell death assay

Apoptotic and necrotic cell populations were evaluated using Annexin V-FITC/propidium iodide (PI). U251 and A172 cells were seeded by a density of 5×10⁴ cells in flasks and incubated under 5% CO2 at 37°C in an incubator until they reached 80% confluence. Cells treated with MYXV at MOIs of 0, 1 and 5 were harvested at 0, 1 and 2 days post-infection, then washed with PBS twice, centrifuged at 805 × g for 5 min and resuspended in 1 ml binding buffer. Following this, cells were stained with 5 µl PI and examined using an Annexin V-FITC apoptosis detection kit (Cell Signaling Technology, Inc., Beverly, MA, USA). Cells were incubated at room temperature in a dark environment for 15 min. The percentage of cell death was assessed by flow cytometry (FCM) using CellQuest software (BD Biosciences, San Jose, CA, USA).

Western blot analysis

To study the effect of MYXV on phosphorylated AKT (p-AKT) in neuroglioma cells, total AKT and p-AKT expression was evaluated in U251 and A172 cells by western blot analysis. Neuroglioma cells were infected with 5 MOI of MYXV, and untreated cells were set as a negative control. After being infected for 0, 1 or 2 days, whole proteins were extracted from cell lysates with RIPA lysis buffer (Beyotime Institute of Biotechnology, Shanghai, China; 50 nM Tris, pH 7.4, 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 0.05 mM EDTA), separated by 10% SDS-PAGE and transferred onto nitrocellulose membranes (Hoffman-La Roche, AG, Basel, Switzerland). The membranes were blocked with 5% skimmed milk in Tris-buffered saline, then incubated with primary polyclonal antibodies anti-AKT (pan; catalogue no. C67E7) at a dilution of 1:1,000 and monoclonal anti-p-AKT (Ser-473; catalogue no. D9E) XP at a dilution of 1:1,000 at 4°C overnight, both purchased from Cell Signaling Technology, Inc (Beverly, MA, USA). Afterwards, the membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated secondary antibody (1:10,000; catalogue no. HAF017; R&D Systems; Bio-Techne, Minneapolis, MN, USA). The bands were visualized using an ECL Western Blotting Kit (BioVision, Inc., Milpitas, CA, USA) and quantified by Quantity One version 4.6.2 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

Statistical analysis

All experiments were repeated a minimum of three times. All data are presented as mean ± standard deviation wherever applicable. GraphPad Prism version 5 software was used to perform statistical analysis. We used a Student's t-test and two-way analysis of variance (ANOVA) to determine significance. P<0.05 was considered to represent statistically significant differences.

Results

MYXV affected cell viability in a dose-dependent manner

Different infective doses of cell lethality were investigated and similar results were found in two human malignant glioma cell lines (U251 and A172). As shown in Fig. 1, both U251 and A172 cells were susceptible to infection by MYXV, which was consistent with a previous study (16). Furthermore, >80% of the U251 and A172 cells were killed by MYXV at an MOI of 10. When the MOI was 5, both U251 and A172 cell lethality was between 40 and 60%, which was determined to be the most suitable infection status for the present study. In addition, the cell viabilities of the U251 and A172 cells that did not receive myxoma virus were used as negative control for all experiments in the present study. Based on this result, an MOI of 5 was used in the subsequent experiments.

Figure 1.

MYXV induces cell death in a dose-dependent manner. U251 and A172 cells were infected with different MOIs of MYXV and their survival rate was detected by MTT assay three days later. MYXV, myxoma virus; MOI, multiplicity of infection.

MYXV affected cell viability in a time-dependent manner

The effect of different incubation periods of MYXV on cell death was determined by MTT assay. Neuroglioma cells were infected with MYXV at MOI of 5. After incubation periods of 0, 1, 2 and 3 days, the survival rates of U251 and A172 were detected by MTT. Cell survival rates were reduced as the length of the incubation period increased, and A172 cells were more sensitive to MYXV than U251 cells (P<0.05; Fig. 2).

Figure 2.

MYXV induces cell death in a time-dependent manner. U251 and A172 cells were infected with MYXV (MOI=5), for periods of 1, 2 or 3 days, after which the cell survival rate was detected by MTT. ***P<0.001 2 days post-infection of U251 cells vs. A172 cells, *P<0.05 3 days post-infection of U251 cells vs. A172 cells. Ns, no statistical significance; MYXV, myxoma virus; MOI, multiplicity of infection.

MYXV induced cell apoptosis in a time- and dose-dependent manner

FCM was conducted to detect the effect of MYXV on cell apoptosis. Neuroglioma cells were infected with MYXV at different MOIs (0, 1 and 5). As shown in Fig. 3A and B, compared with MOI 0, the proportion of apoptotic cells in U251 and A172 cells increased at MOI 5, which indicated that both U251 and A174 cells were susceptible to being killed by MYXV in a dose-dependent manner (P<0.05; Fig. 3A and B). Furthermore, the percentage of apoptotic cells in U251 and A172 after treatment with 5 MOI MYXV for 0, 1 and 2 days were calculated (Fig. 3C). With the increase of time post-infection, cell apoptosis rate of U251 and A172 cells also increased compared to day 0 (P<0.05; Fig. 3C), which suggested that MYXV induced cell apoptosis in a time-dependent manner.

Figure 3.

Effect of MYXV on cell death in different incubation periods. (A) Representative dot plot of cell population distribution stained for Annexin V-FITC/PI at MOIs of 0, 1 and 5. (B) Percentage of apoptotic cells in U251 and A172 when treated with 5 MOI MYXV for 0, 1 and 2 days. *P<0.05 vs. 0 days. MYXV, myxoma virus; PI, propidium iodide; MOI, multiplicity of infection.

Effect of MYXV on p-AKT activity

The levels of p-AKT are directly involved in the susceptibility of tumor cells to infection with MYXV (9,17). To examine whether similar effects are observed in U251 and A172 cell lines, western blot analysis was used to detect the expression levels of total AKT and p-AKT in U251 and A172 cells that had been infected by MYXV at MOI 5 for 0, 1 and 2 days. Consistent with previous studies, MYXV induced the phosphorylation of AKT in both U251 and A172 cell lines in a time-dependent manner (Fig. 4). Then a densitometric analysis was performed on these results by Quantity One version 4.6.2 software. According to the densitometric analysis, in U251 cells, there was a significant increase in the expression levels of p-AKT on day 2 compared with day 0 (P<0.05; Fig. 4B) and in A172 cells, there was a significant increase in p-AKT expression levels on day 1 and 2 compared to day 0 (P<0.05; Fig. 4B).

Figure 4.

Effect of MYXV on p-AKT activity in U251 and A172 cells. (A) Western blot analysis was used to detect total AKT and p-AKT in U251 and A172 cells 0, 1 and 2 days post-MYXV infection. (B) Relative p-AKT protein level in U251 and A172 cells after 0, 1 and 2 days. ***P<0.001 1 or 2 days post-infection vs. 0 days, **P<0.01 2 vs. 1 days post-infection of A172 cells or 1 vs. 0 days post-infection of A172 cells. Ns, no statistical significance; MYXV, myxoma virus; p-AKT, phosphorylated AKT.

Discussion

Malignant gliomas are the most common and aggressive primary central nervous system tumor in humans. Numerous types of conventional pro-apoptotic therapies have been applied to resistant malignant gliomas, such as radiotherapy, chemotherapy and adjuvant therapies, but these have not been effective in killing glioma cells (18,19). It is therefore crucial to develop an original approach for malignant glioma therapy. Oncolytic virotherapy, a novel and promising cancer therapeutic strategy, is reported to be more effective and have fewer side effects than conventional cancer therapies (20–22), and has previously been investigated as a treatment for gliomas (23). Candidate oncolytic viruses should produce few side-effects, be non-pathogenic and exhibit selective anti-tumor activities.

MYXV belongs to the poxviridae family. It is a rabbit-specific virus and exhibits restricted pathogenicity for all other vertebrate species, including humans (24,25). Despite its limited host range specificity, MYXV has been shown to selectively infect diverse forms of human tumor cells, including glioma cells (8). In the present study, MYXV acted as a vital factor that affected cell survival rates. In addition, MYXV exerted such effects in a dose and time-dependent manner. The results also indicated that MYXV contributed to apoptosis in human neuroglioma cell lines U251 and A172 in a dose and time-dependent manner.

Notably, MYXV exerts a selective tropism for tumor cells with elevated levels of p-AKT (8). AKT, also known as protein kinase B, is a serine/threonine protein kinase which plays an important role in cell survival and apoptosis (17). AKT phosphorylates a series of proteins and inhibits apoptosis via a number of mechanisms (26). It has been confirmed that the majority of both malignant and recurrent glioma cells exhibit PTEN gene inactivation or deletion, which increases AKT activity and results in cell proliferation and inhibition of apoptosis. Data from the current study indicated that the expression levels of p-AKT significantly increased in MYXV-infected U251 and A172 cell lines compared with cell lines receiving an inactive virus.

Furthermore, the present results verified the stated association between the activation of AKT and MYXV-mediated oncolysis in vitro. This highlights the potential oncolytic function of MYXV on human glioma cells and provides a promising therapeutic target for human malignant glioma tumors. Further studies are required to verify whether MYXV can promote oncolysis through modulating the levels of activated AKT within other types of tumor cell in vitro, which could make MYXV a key factor in improving the outcome of treatment for various cancer types.