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. 2017 May 30;33(25):6342–6352. doi: 10.1021/acs.langmuir.7b00527

Figure 4.

Figure 4

Strain-stiffening behavior of fibrin networks formed at different fibrin and HEPES concentrations. The differential elastic modulus, K′, was measured using a prestress protocol and plotted against the applied prestress, σ, for 1 (circles), 3 (squares), and 6 mg/mL (triangles) fibrin samples formed with (A) 20 (blue), (B) 100 (green), and (C) 200 mM (red) HEPES. (D–F) To reveal the force–extension behavior of the individual protofibrils, data were rescaled by the protofibril density, ρpf (total protofibril length per volume). Observed collapse of the curves at forces above 10 pN independent of HEPES (and fibrin) concentration indicates that HEPES does not alter the intrinsic force–extension behavior of the protofibrils.