Figure 7.

Multiplex analysis of phosphorylated proteins of NF-κB pathway in curcumin pre-treated HT29 cells. HT29 cells were pre-treated with curcumin (Cur) and then, after 24 h, stimulated with IFNγ for a further 48 h to detect changes in phosphorylated proteins belonging to the NF-κB pathway (NF-κB, IKKα/β, and IκBα), using a magnetic bead signaling kit and a Bio-Plex multiplex reader. Data, expressed as the median fluorescence intensity (MFI), represent the mean ± SD of four replicates and were analyzed with one-way ANOVA using the Bonferroni post-test (b: p < 0.01; c: p < 0.001).