Abstract
Fourier-transform infrared spectroscopy (FT-IR) was applied to the study of tissue sections of human colorectal cancer. Pairs of tissue samples from colorectal cancer and histologically normal mucosa 5-10 cm away from the tumor were obtained from 11 patients who underwent partial colectomy. All cancer specimens displayed abnormal spectra compared with the corresponding normal tissues. These changes involved the phosphate and C-O stretching bands, the CH stretch region, and the pressure dependence of the CH2 bending and C = O stretching modes. Our findings indicate that in colonic malignant tissue, there are changes in the degree of hydrogen-bonding of (i) oxygen atoms of the backbone of nucleic acids (increased); (ii) OH groups of serine, tyrosine, and threonine residues (any or all of them) of cell proteins (decreased); and (iii) the C = O groups of the acyl chains of membrane lipids (increased). In addition, they indicate changes in the structure of proteins and membrane lipids (as judged by the changes in their ratio of methyl to methylene groups) and in the packing and the conformational structure of the methylene chains of membrane lipids. The cell(s) of the malignant colon tissues responsible for these spectral abnormalities is unknown. Cultured colon adenocarcinoma cell lines displayed similarly abnormal FT-IR spectra. The diagnostic potential of the observed changes is discussed.
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- ASTLER V. B., COLLER F. A. The prognostic significance of direct extension of carcinoma of the colon and rectum. Ann Surg. 1954 Jun;139(6):846–852. doi: 10.1097/00000658-195406000-00015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Auger M., Jarrell H. C., Smith I. C., Wong P. T., Siminovitch D. J., Mantsch H. H. Pressure-induced exclusion of a local anesthetic from model and nerve membranes. Biochemistry. 1987 Dec 29;26(26):8513–8516. doi: 10.1021/bi00400a003. [DOI] [PubMed] [Google Scholar]
- Bishop J. M. Viral oncogenes. Cell. 1985 Aug;42(1):23–38. doi: 10.1016/s0092-8674(85)80098-2. [DOI] [PubMed] [Google Scholar]
- Fossel E. T., Carr J. M., McDonagh J. Detection of malignant tumors. Water-suppressed proton nuclear magnetic resonance spectroscopy of plasma. N Engl J Med. 1986 Nov 27;315(22):1369–1376. doi: 10.1056/NEJM198611273152201. [DOI] [PubMed] [Google Scholar]
- McDonald R. S. Infrared spectrometry. Anal Chem. 1986 Aug;58(9):1906–1925. doi: 10.1021/ac00122a003. [DOI] [PubMed] [Google Scholar]
- Peeling J., Sutherland G., Marat K., Tomchuk E., Bock E. 1H and 13C nuclear magnetic resonance studies of plasma from patients with primary intracranial neoplasms. J Neurosurg. 1988 Jun;68(6):931–937. doi: 10.3171/jns.1988.68.6.0931. [DOI] [PubMed] [Google Scholar]
- Shulman R. NMR--another cancer-test disappointment. N Engl J Med. 1990 Apr 5;322(14):1002–1003. doi: 10.1056/NEJM199004053221410. [DOI] [PubMed] [Google Scholar]
- Wong P. T., Huang C. H. Structural aspects of pressure effects on infrared spectra of mixed-chain phosphatidylcholine assemblies in D2O. Biochemistry. 1989 Feb 7;28(3):1259–1263. doi: 10.1021/bi00429a046. [DOI] [PubMed] [Google Scholar]
- Wong P. T., Mantsch H. H. Reorientational and conformational ordering processes at elevated pressures in 1,2-dioleoyl phosphatidylcholine: a Raman and infrared spectroscopic study. Biophys J. 1988 Nov;54(5):781–790. doi: 10.1016/S0006-3495(88)83016-9. [DOI] [PMC free article] [PubMed] [Google Scholar]