Figure 5. CRISPR alters population structure in vitro in a two-strain competition.
Conjugation competition assays were performed with CK111SSp(pCF10-101) and V583 with (A) pKH12 and (B) pKHS67 using a 1/1000 dilution of the initial mating reaction for the first chloramphenicol passage. Experiments with CK111SSp(pCF10-101) and V649 were simultaneously performed with (C) pKH12 and (D) pKHS67. ‘X’ values indicate those that fell below the threshold of detection. Geometric mean and standard deviation are shown for 3 independent biological replicates. V583 and V649 genotypes have been shown again for clarity.
DOI: http://dx.doi.org/10.7554/eLife.26664.012
Figure 5—source data 1. Replicate values for growth curves.
OD600nm values for each individual replicate are provided, which are shown in Figure 5—figure supplement 1.
elife-26664-fig5-data1.xlsx (41.1KB, xlsx)
DOI: 10.7554/eLife.26664.013
Figure 5—figure supplement 1. CRISPR targeting results in growth defect in chloramphenicol.
Average growth curves from three colonies in triplicate (for pCR2 experiments) or in duplicate (for pKH12 experiments) are shown. Error bars are omitted for groups that lack CRISPR targeting for clarity.
Figure 5—figure supplement 2. CRISPR alters population structure in vitro in a two-strain competition at higher initial dilutions.
Conjugation competition assays were performed with CK111SSp(pCF10-101) and V583 with (A) pKH12 and (B) pKHS67 with a 1/10,000 dilutions of the mating reaction (as opposed to 1/1000 in Figure 7). Experiments with CK111SSp(pCF10-101) and V649 were simultaneously performed with (C) pKH12 and (D) pKHS67. ‘X’ values indicate those that fell below the threshold of detection. Geometric mean and standard deviation are shown for 4 and 3 independent biological replicates.