Figure 8. CRISPR targeting removes specific antibiotic resistances from populations in vitro.
(A) Genotypes of select strains and plasmids are shown. (B–C) Conjugation-competition assays are shown, similar to those in Figure 7. T11CR1 carrying pAM771 was used, but transconjugants were selected on only rifampicin, fusidic acid, and chloramphenicol to assess if T11CR1 was able to survive with the loss of pAM771. V649 possesses ermB on pTEF1. Total ermR CFU accounts for both V649 and T11RF+pAM771. ‘X’ indicate values that fell below the threshold of detection.
Figure 8—figure supplement 1. CRISPR targeting and two-strain competition can remove antibiotic resistances in vitro.
CK111SSp(pCF10-101) carrying (A) pCR2 or (B) pCR2-ermB was conjugated into V649 and the reaction was passaged in chloramphenicol overnight. ‘X’ values indicate those that fell below the threshold of detection. The geometric mean and geometric standard deviation are shown for two independent biological replicates.