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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Small. 2017 Feb 14;13(16):10.1002/smll.201603847. doi: 10.1002/smll.201603847

Figure 3.

Figure 3

Effects of SNA human serum protein corona formation on macrophage uptake. A) The uptake and association of G-rich and poly-T SNAs in the presence and absence of HS following 15–90 min incubations with THP-1 cells was quantified by ICP-MS. G-rich SNAs exhibit significantly higher uptake in the presence of HS compared to poly-T SNAs with or without HS present. B) The uptake of SNAs into THP-1 macrophages following treatment with chemical blockers to inhibit binding to cell surface receptors was quantified. Fucoidan was used to block class A scavenger receptors, Fc block was used to block Fc receptors, and trypan blue was used to block complement receptors. The results indicate that the macrophage uptake and association of SNAs in the presence of HS was significantly inhibited by trypan blue and suggests that complement receptor-mediated phagocytosis plays a large role in the uptake of protein-coated SNAs into THP-1 macrophage cells. *P < 0.05.