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. Author manuscript; available in PMC: 2017 Jun 30.
Published in final edited form as: Methods. 2016 Oct 26;113:64–71. doi: 10.1016/j.ymeth.2016.10.013

Fig. 6.

Fig. 6

Evaluation of threonine and borrelidin binding by ThrRS (A) ThrRS (blue circles) fluorescence thermal melting data as a function of temperature shown with threonine binding (empty orange squares) and BN binding (filled red triangles) (B) Fluorescence data converted to first derivative plot. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)