Fig. 6.

Differentiation of FXN siRNA transfected SH-SY5Y cells. a Representative images of SH-SY5Y H₂O carrier transfected controls (Control) cultured for 8 days with or without addition of retinoic acid (RA). b Images depicting cells with either an N-type or S-type morphology. c The number N-type cells, S-type cells and the S-type/N-type ratio following transfection with H₂O carrier (control) or FXN siRNA and differentiation with RA with/without exposure to MSC conditioned medium (MSC). d Representative images of RA differentiated SH-SY5Y cells following transfection with FXN siRNA with/without exposure to MSC conditioned medium (MSC). Red arrows indicate the position of S-type cells. ± SEM; n = 5; *p < 0.05; ns not significant; Scale bars = 100 μm (a, d), 50 μm (b)