Figure 3.

Examples of expression systems used for recombinant expression of antitumour IgE. (A) Recombinant IgE by cloning the variable domains of IgG of desired specificity to an IgE constant domain. (B) Classical restriction enzyme‐based cloning requires the presence of specific restriction sites flanking the gene of interest. (C) Expression of IgE by insect cells requires a recombinant baculovirus stock containing the antibody expression cassette. (D) Polymerase Incomplete Primer Extension (PIPE) cloning facilitates a rapid cloning of DNA sequences with the option of performing site‐specific mutagenesis at the same time.