Table 6. rad9Δ instabilities using different chemical growth media.

	Genotype	Unstable Chr. (×10−5)		Allelic rec. (×10−5)		Chr. loss (×10−4)
		Median (Q1, Q3)	Fold stabil.	Median (Q1, Q3)	Fold stabil.	Median (Q1, Q3)	Fold stabil.
	rad9Δ	57 (40, 71)	1	6.0 (2.8, 8.2)	1	22 (16, 39)	1
Cycloheximide	rad9Δ 0.04 μg/ml cyc.	78 (67, 99)	0.73	8.4 (4.6, 12)	0.71	47 (28, 57)	0.47
	rad9Δ 0.06 μg/ml cyc.	86 (67, 97)	0.66	6.4 (3.1, 12)	0.94	36 (19, 53)	0.61
D-glucosamine	rad9Δ 2% Dex, 0.2% D-gluc	62 (54, 90)	0.92	2.2 (1.1, 4.8)	2.7	6.9 (6.0, 8.2)	3.2*
	rad9Δ 2% Dex, 0.7% D-gluc	72 (43, 130)	0.79	9.0 (8.1, 9.3)	0.67	11 (7.1, 16)	2.0
	rad9Δ 2% Dex, 1.0% D-gluc	33 (23, 42)	1.7	1.1 (0.28, 4.3)	5.5*	30 (14, 51)	0.73
	rad9Δ 2% Dex, 1.8% D-gluc	27 (19, 30)	2.1*	3.3 (2.2, 5.9)	1.8	14 (8.8, 19)	1.6
Myriocin	rad9Δ 800 ng/ml myriocin	59 (52, 71)	0.97	7.0 (4.7, 10)	0.86	10 (8.7, 16)	2.2*
	rad9Δ 1000 ng/ml myriocin	43 (36, 50)	1.3	2.2 (1.4, 3.0)	2.7*	5.8 (4.9, 8.1)	3.8*
NaCl	rad9Δ 0.125 M NaCla	33 (27, 36)	1.7	2.5 (2.0, 7.5)	2.4	21 (12, 27)	1.00
	rad9Δ 0.25 M NaCla	44 (34, 55)	1.3	13 (11, 17)	0.46*	92 (60, 105)	0.24
	rad9Δ 0.5 M NaCla	47 (35, 54)	1.2	12 (10, 14)	0.50*	76 (67, 130)	0.30*
	rad9Δ 1.0 M NaCla	120 (94, 180)	0.48*	9.0 (5.5, 23)	0.67	4900 (3900, 6300)	0.0045*
YEPG	rad9Δ 2% glycerolb	29 (15, 31)	2.0	2.5 (1.4, 6.1)	2.4	710 (350, 810)	0.031*

Instability frequencies and genome fold stabilization of rad9Δ grown on media plates containing various concentrations of cycloheximide (protein synthesis inhibitor), D-glucosamine (glucose competitor), myriocin (sphingolipid inhibitor), NaCl (osmotic stress inducer), and YEPG (glycerol, respiration energy source) normalized to rad9Δ grown on YEPD media plates. Cells with a light gray background indicate genome fold stabilization increase; cells with a white background indicate decreased fold stabilization (<1.0 = increased instability) or no change in stabilization (=1.0). Statistically significant appears in boldface type. Kruskal–Wallis test, * P < 0.01.

^aViability not tested.

^bSample size: N = 5.