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. 2017 Apr 20;16(11):1063–1069. doi: 10.1080/15384101.2017.1314406

Combination of gemcitabine and docetaxel regresses both gastric leiomyosarcoma proliferation and invasion in an imageable patient-derived orthotopic xenograft (iPDOX) model

Kei Kawaguchi a,b,c, Kentaro Igarashi a,b, Takashi Murakami a,b, Tasuku Kiyuna a,b, Scott D Nelson d, Sarah M Dry d, Yunfeng Li d, Tara A Russell e, Arun S Singh f,g, Bartosz Chmielowski f, Michiaki Unno c, Fritz C Eilber e,g,✉, Robert M Hoffman a,b,g,✉
PMCID: PMC5499841  PMID: 28426279

ABSTRACT

Gastric leiomyosarcoma is a recalcitrant cancer and the chemotherapy strategy is controversial. The present study used a patient-derived orthotopic xenograft (PDOX) nude mouse model of gastric leiomyosarcoma to identify an effective therapeutic regimen to develop individualized precision medicine for this disease. The gastric leiomyosarcoma obtained from a patient was first grown in transgenic nude mice ubiquitously expressing red fluorescent protein (RFP) to stably label the tumor stroma. The RFP-expressing tumor was then passaged orthotopically in the gastric wall of non-transgenic nude mice to establish an imageable PDOX (iPDOX) model. The bright fluorescent tumor was readily imaged over time to determine drug efficacy. Four weeks after implantation, 70 PDOX nude mice were divided into 7 groups: control without treatment (n = 10); doxorubicin (DOX) (2.4 mg/kg, intraperitoneally (i.p.), once a week for 2 weeks, n = 10); gemcitabine (GEM)/ docetaxel (DOC) (GEM: 100 mg/kg, DOC: 20 mg/kg, i.p., once a week for 2 weeks, n = 10); cyclophosphamide (CPA) (140 mg/kg, i.p., once a week for 2 weeks, n = 10); temozolomide (TEM) (25 mg/kg, orally, daily for 14 consecutive days, n = 10); yondelis (YON) (0.15 mg/kg, i.v., once a week for 2 weeks, n = 10); pazopanib (PAZ) (100 mg/kg, orally, daily for 14 consecutive days, n = 10). On day 14 from initiation of treatment, all treatments except PAZ significantly inhibited tumor growth compared with untreated control (DOX: p < 0.01, GEM/DOC: p < 0.01, CPA: p < 0.01, TEM: p < 0.01, YON: p < 0.01) on day 14 after initiation. In addition, only GEM/DOC was more significantly effective than DOX (p < 0.05). GEM/DOC could regress the leimyosarcoma in the PDOX model and has important clinical potential for precision individual treatment of leiomyosarcoma patients.

KEYWORDS: docetaxel, drug-response, gemcitabine, gastric leiomyosarcoma, nude mice, orthotopic, PDOX, precision therapy, red fluorescent protein, tumor regression

Introduction

Leiomyosarcoma is the most common histotype of gastro-intestinal sarcoma.1 Gastro-intestinal leiomyosarcoma originates from smooth muscle or a blood vessel2 and is a recalcitrant disease, relatively unresponsive to chemotherapy or radiation. Currently only surgery is curative2 and 50% of patients will develop local recurrence after tumor resection and subsequently distant metastases.3

Doxorubicin (DOX) and ifosfamide (IFO) are first line for sarcomas but not effective for leiomyosarcoma with a response rate of only 18%.4 Gemcitabine (GEM) and docetaxel (DOC) have efficacy for adjuvant treatment of uterine leiomyosarcoma,5 but efficacy has not been shown for gastrointestinal leiomyosarcoma. Yondelis (YON) has shown efficacy for leiomyosarcoma of both uterine and non-uterine origin,6 but not yet for gastrointestinal leiomyosarcoma. The tyrosine kinase inhibitor pazopanib (PAZ) has not demonstrated significant activity against leiomyosarcoma.7 Leiomysarcoma is heterogeneous as well as treatment resistant. Novel more effective personalized therapy is therefore needed for leiomysarcoma.

Clinically-relevant mouse models of leiomyosarcoma could enable both discovery of new treatment strategies as well as individualized precision therapy, based on each patient's tumor. Toward this goal, our laboratory pioneered the patient-derived orthotopic xenograft (PDOX) nude mouse model with the technique of surgical orthotopic implantation (SOI), including pancreatic,8-11 breast,12 ovarian,13 lung,14 cervical,15 colon,16-18 stomach,19 sarcoma,20-24 and melanoma.25-27 The PDOX model, developed by our laboratory over the past 29 years, has many advantages over subcutaneous-transplant models which are growing ectopically under the skin.28

Our laboratory has also developed the imageable PDOX (iPDOX) model using fluorescent protein labeling. The patient tumors were passaged orthotopically in transgenic nude mice ubiquitously expressing red fluorescent protein (RFP) or green fluorescent protein (GFP) or cyan fluorescent protein (CFP). The primary patient tumors acquired RFP-expressing stroma.29 Subsequent liver metastases and disseminated peritoneal metastases maintained the stroma from the primary tumor, and possibly recruited additional fluorescent protein-expressing stroma, resulting in their very bright fluorescence.30 Tumors, which acquired very bright fluorescent protein-expressing stromal cells, were then orthotopically passaged to non-transgenic nude mice. It was possible to image the brightly fluorescent tumors non-invasively longitudinally as they progressed in the non-transgenic nude mice.31 Undifferentiated pleomorphic sarcoma (UPS) PDOX tumors, previously grown in RFP transgenic nude mice for only one passage, had very bright fluorescence and after passage to non-transgenic nude mice maintained the bright fluorescence and were non-invasively imageable.,32,33

A telomerase-dependent GFP-containing adenovirus OBP-401 was used to label the cancer cells of a pancreatic cancer PDOX which was previously grown in a RFP transgenic mouse that stably labeled the PDOX stroma cells bright red. This dual-color PDOX model was shown to be very effective for fluorescence-guided surgery (FGS).34

In the present study, we established a PDOX nude mouse model with gastric leiomyosarcoma from a patient and evaluated the efficacy of 6 drugs to find an effective treatment strategy against leiomyosarcoma tumor growth and metastasis.

Results and discussion

All treatments except for PAZ significantly inhibited tumor volume compared with untreated control; DOX: p < 0.01; GEM/DOC: p < 0.01; CPA: p < 0.01; TEM: p < 0.01; YON: p < 0.01; on day 14 after initiation. GEM/DOC was significantly more effective than DOX, a first-line therapy for sarcoma (p < 0.01) (Fig. 1, Fig. 2).

Figure 1.

Figure 1.

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Imaging of drug efficacy on a leiomyosarcoma iPDOX. The PDOX model treated with GEM combined with DOC (GEM/DOC) demonstrated tumor regression. Scale bar: 5 mm.

Figure 2.

Figure 2.

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Efficacy of treatment on leiomyosarcoma iPDOX. Bar graph shows relative tumor volume at a post-treatment point relative to the initial pre-treatment tumor volume. All treatments except for PAZ significantly inhibited tumor growth compared with untreated control (CTR). GEM/DOC was the strongest and significantly more effective than other therapies (DOX: p < 0.01, CPA: p < 0.01, TEM: p < 0.01, YON: p < 0.01, PAZ: p < 0.01). **p < 0.01. Error bars: ± SD.

The leiomyosarcoma was grown in red fluorescent protein (RFP) transgenic mice whereby it stably acquired RFP-expressing stroma.31,32

Treatment efficacy in gastric leiomyosarcoma PDOX was evaluated by tumor fluorescent area and tumor fluorescence intensity. As shown in Figure 3, all treatments could significantly reduce both fluorescent tumor area and tumor fluorescence intensity compared with untreated control: fluorescent area = 72.1 ± 44.3 (mm3); fluorescence intensity = 776644 ± 418682; DOX: fluorescent area = 22.1 ± 17.3 (mm3), p < 0.01; fluorescence intensity = 256531 ± 226593, p < 0.01; GEM/DOC: fluorescent area = 8.84 ± 1.23 (mm3), p <0.01; fluorescence intensity = 139885 ± 12081, p < 0.01; CPA: fluorescent area = 19.8 ± 18.0 (mm3), p < 0.01; fluorescence intensity = 233161 ± 175932, p < 0.01; TEM: fluorescent area = 22.6 ± 15.8 (mm3), p < 0.01; fluorescence intensity = 308721 ± 215154, p <0.01; YON: fluorescent area = 16.3 ± 10.1 (mm3), p<0.01; fluorescence intensity = 216880 ± 145555, p < 0.01; PAZ: fluorescent area = 32.0 ± 17.2 (mm3), p < 0.05; fluorescence intensity = 396556 ± 306308, p < 0.05. There was a strong correlation between tumor fluorescent area and fluorescence intensity (R2 = 0.9105, p <0.0001) (Fig. 4).

Figure 3.

Figure 3.

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Treatment efficacy for leiomyosarcoma iPDOX. Bar graphs show the tumor fluorescent area (mm2) (A) and fluorescence intensity (B). All treatments significantly inhibited the leiomyosarcoma iPDOX compared with untreated control. **p < 0.01, *p < 0.05. Error bars: ± SD.

Figure 4.

Figure 4.

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Correlation of tumor fluorescent area and tumor fluorescence intensity. Tumor area significantly correlated with fluorescence intensity (R2 = 0.9105, p < 0.0001). Please see Materials and methods for details.

Tumor volume at post-treatment, relative to the initial pre-treatment tumor volume, is shown below: untreated control: 3.75 ± 1.63; DOX: 0.78 ± 0.28; GEM/DOC: 0.39 ± 0.09; CPA: 0.51 ± 0.23; TEM: 0.56 ± 0.28; YON: 1.08 ± 0.34; PAZ: 1.96 ± 0.75. Both relative tumor fluorescent area and relative fluorescence intensity showed a strong positive correlation with relative tumor volume (R2 = 0.8927, p <0.0001: R2 = 0.8967, p < 0.0001: respectively) (Fig. 5).

Figure 5.

Figure 5.

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Correlation of fluorescence intensity and fluorescent area with tumor volume. Both relative tumor fluorescent area (A) and relative fluorescence intensity (B) demonstrated a strong positive correlation with relative tumor volume (R2 = 0.8927, p < 0.0001; R2 = 0.8967, p < 0.0001; respectively).

The relative body weight on day 14 compared with day 0 did not significantly differ between each treatment group, including untreated control (Fig. 6).

Figure 6.

Figure 6.

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Effect of each treatment on mouse body weight. Bar graph shows relative body weight at post-treatment relative to the pre-treatment. There were no significant differences between each treatment group including untreated control.

Histologically, the untreated control tumor was mainly comprised of viable cells without obvious necrosis. In contrast, the tumor treated with GEM/DOC had extensive necrosis (Fig. 7).

Figure 7.

Figure 7.

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Histological response. A. The untreated control comprised viable cells without necrosis. B. The tumor treated with GEM and DOC exhibited necrosis. Scale bars: 100 μm.

The DOX response rate in the clinic for leiomyosarcoma was reported as only 18%,3 however, in the present study, DOX was effective for the gastric leiomyosarcoma PDOX. YON has shown efficacy for many types of leiomyosarcoma,6 however, its treatment efficacy was less than GEM/DOC which showed the most efficacy in the present study.

The present study took advantage of technology we previously developed to stably label the stroma of a patient tumor by growing it in transgenic mice ubiquitously-expressing a fluorescent protein, in this case, RFP. The tumor was then passaged to non-transgenic mice which enabled high contrast imaging that showed the invasive capability of the tumor which was inhibited by effective chemotherapy. We have termed this model imageable PDOX (iPDOX) which should be highly useful for imaging tumor growth, invasion and metastasis.

Therefore, the PDOX model may be useful for precise individualized therapy, especially for rare heterogeneous sarcomas such as gastric leiomyosarcoma.26

Previously-developed concepts and strategies of highly selective tumor targeting can take advantage of molecular targeting of tumors, including tissue-selective therapy which focuses on unique differences between normal and tumor tissues.35-40

Conclusions

In the present study, the iPDOX model helped identify the high efficacy of GEM combined with DOC against the gastric leiomyosarcoma. GEM/DOC caused tumor regression and arrested the invasiveness of the tumor. The PDOX model for gastric leiomyosarcoma will be further developed to identify effective individualized patient therapy.

Materials and methods

Mice

Athymic non-transgenic nu/nu nude mice and transgenic nude mice ubiquitously-expressing red fluorescent protein (RFP) mice (AntiCancer Inc., San Diego, CA), 4–6 weeks old, were used in this study.41 The transgenic nude mice express the RFP gene under the control of the chicken β-actin promoter and cytomegalovirus enhancer.42 Animals were housed in a barrier facility on a high efficacy particulate arrestance (HEPA)-filtered rack under standard conditions of 12-hour light/dark cycles. The animals were fed an autoclaved laboratory rodent diet. All mouse surgical procedures and imaging were performed with the animals anesthetized by subcutaneous injection of a ketamine mixture (0.02 ml solution 20 mg/kg ketamine, 15.2 mg/kg xylazine, and 0.48 mg/kg acepromazine maleate). The response of animals during surgery was monitored to ensure adequate depth of anesthesia. The animals were observed on a daily basis and humanely killed by CO2 inhalation if they met the following humane end point criteria: severe tumor burden (more than 20 mm in diameter), prostration, significant body weight loss, difficulty breathing, rotational motion and body temperature drop. All animal studies were conducted in accordance with the principles and procedures outlined in the National Institutes of Health Guide for the Care and Use of Animals under Assurance Number A3873–1.

Patient-derived tumor

A patient diagnosed with gastric leiomyosarcoma had the tumor resected in the Department of Surgery, University of California, Los Angeles (UCLA). Written informed consent was provided by the patient, and the Institutional Review Board (IRB #10–001857) of UCLA approved this experiment. The patient had a highly-aggressive and high-grade gastric leiomyosarcoma that had grown to greater than 10 cm and was resected from the gastric wall in the upper abdomen. After the original tumor resection that was used for the PDOX model, the patient recurred with diffuse regional metastasis 4 months after surgery.

Establishment of PDOX models of leiomyosarcoma by surgical orthotopic implantation (SOI)

A fresh sample of gastric leiomyosarcoma of the patient was obtained and transported immediately to the laboratory at AntiCancer, Inc., on wet ice. The sample was cut into 5-mm fragments and implanted subcutaneously in nude mice. After 3 weeks, the subcutaneously-implanted tumors grew to more than 10 mm in diameter. The subcutaneously-grown tumors were cut into 5-mm fragments and implanted subcutaneously in RFP transgenic nude mice.41-43 After 3 weeks, the subcutaneously-implanted tumors in RFP mice were harvested and cut into small fragments (3 mm3). After non-transgenic nude mice were anesthetized with the ketamine solution described above, a 1–2 cm skin incision was made on the middle abdomen through the skin, fascia and peritoneum and thereby the stomach was exposed. Surgical sutures (8–0 nylon) were used to implant tumor fragments onto the gastric wall to establish the PDOX model. The wound was closed with a 6–0 nylon suture (Ethilon, Ethicon, Inc., NJ, USA).44

Treatment study design in the leiomyosarcoma PDOX model

PDOX mouse models were randomized into 7 groups of 10 mice each: untreated control; DOX (2.4 mg/kg, i.p., qw × 2); GEM/DOC (GEM: 100 mg/kg, DOC: 20 mg/kg, i.p., qw × 2); CPA (140 mg/kg, i.p., qw × 2); TEM (25 mg/kg, p.o., qd × 14); YON (0.15 mg/kg, i.v., qw × 2); PAZ (100 mg/kg, p.o., qd × 14). Tumor length and width were measured after 14 d treatment. Tumor volume was calculated with the following formula: Tumor volume (mm3) = length (mm) × width (mm) × width (mm) × 1/2. Data are presented as mean ± SD. The tumor volume ratio is defined as the tumor volume at a post-treatment point relative to pre-treatment tumor volume.

Imaging of the fluorescent leiomyosarcoma PDOX model

Fluorescence imaging of the macroscopic tumor was performed with the OV100 Small Animal Imaging System (Olympus, Tokyo, Japan).45 The tumor fluorescent area and total fluorescence intensity were analyzed with UVP software (UVP, Upland, CA). Treatment efficacy was evaluated by tumor fluorescent area and tumor fluorescence intensity at post-treatment compared with at pre-treatment.46

Histological examination

Fresh tumor samples were fixed in 10% formalin and embedded in paraffin before sectioning and staining. Tissue sections (5 μm) were deparaffinized in xylene and rehydrated in an ethanol series. Hematoxylin and eosin (H&E) staining was performed according to standard protocols. Histological examination was performed with a BHS System Microscope (Olympus Corporation, Tokyo, Japan). Images were acquired with INFINITY ANALYZE software (Lumenera Corporation, Ottawa, Canada).26,27

Statistical analysis

JMP version 11.0 was used for all statistical analyses. Significant differences for continuous variables were determined using the Mann-Whitney U test. Line graphs expressed average values and error bar showed SD. A probability value of P ≤ 0.05 was considered statistically significant.

Disclosure of potential conflicts of interest

No potential conflicts of interest were disclosed.

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