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. 2017 May 11;206(3):1479–1493. doi: 10.1534/genetics.116.196881

Figure 2.

Figure 2

Notching wing phenotypes in wing-specific Psn and Nct KD flies. (A) Expression of shPsn or shNct under the control of the c96-Gal4 driver results in thickened veins and/or notching wing margins. Representative adult wing images of control (c96-Gal4/+) and wing-specific Psn (c96 > shPsn) or Nct (c96 > shNct) KD flies are shown. The wing margin is also shown in higher magnification views. Red dots mark thickened veins or blistered phenotypes in the wing margins. Bar, 0.5 mm. (B) Representative adult wing images used to establish the scoring system for quantifying the severity of the wing phenotypes. Score 0: wild-type wing morphology. Score 1: mildly thickened L3 and L4 veins near the margin. Score 2: thickened L2, L3, L4, and L5 veins near the margin. Score 3: more enhanced vein phenotypes. Score 4: severe vein and blistered phenotypes. Score 5: severe margin loss. (C) Quantification of the severity of wing phenotypes using the scoring system shown in (B). At least 20 wings were scored per genotype. All data are expressed as mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc comparisons, ****P < 0.0001.