Table 1. Generation of genetically targeted SRY-sp1-KO rabbits using CRISPR/Cas9 system.
| Recipients | gRNA/Cas9 mRNA(ng/uL) | Embryos injected | Embryos transferred (%microinjected) |
Pregnancy | Pups obtained (%transferred) |
Pregnancy (%pups) |
Pups obtained (%male pups) |
Pups with Sex reversal |
|---|---|---|---|---|---|---|---|---|
| 1 | 25/100 | 50 | 42(84%) | NO | ||||
| 2 | 25/100 | 45 | 40(89%) | YES | 6(15%) | 2(33.3%) | 2(100%) | 2 |
| 3 | 25/100 | 48 | 40(83.3%) | NO | ||||
| 4 | 25/100 | 45 | 42(93.3%) | YES | 4(9.52%) | 2(50%) | 2(100%) | 1 |
| 5 | 25/100 | 53 | 48(93%) | YES | 3(6.25%) | 2(66.7%) | 2(100%) | 1 |
| 6 | 25/100 | 48 | 45(93.75%) | NO | ||||
| 7 | 25/100 | 52 | 50(96.1%) | YES | 3(6%) | 1(33.3%) | 1(100%) | 1 |
| 8 | 25/100 | 50 | 48(96%) | YES | 4(8.3%) | 2(50%) | 2(100%) | 2 |