Figure 6. Role of histone acetylation in the regulation of CXCL12 gene expression and functional study.

(A) Representative experiment of butyrate- and VPA-induced histone H3 acetylation as quantified by western blotting in SW480, HCT116 and TC7 cell lines. (B) ChIP assays were performed on colon cells treated with but (10 mM) or VPA (1 mM) for 24 h, and the levels of the CXCL12 promoter in the immunoprecipitates were measured by RT-qPCR in SW480, HCT116 and TC7 cell lines.