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. 2017 Mar 24;8(24):39012–39020. doi: 10.18632/oncotarget.16546

Figure 2. Abce1 RNAi delays the resumption of meiosis (GVBD) and affects first polar body extrusion.

Figure 2

Oocytes injected with control or Abce1 siRNA were incubated in M2 medium containing 50 μM IBMX for 24 h and then released into IBMX-free M16 medium for continuously culture. (A) Western blot showing the reduced expression of Abce1 after siRNA injection. Abce1 relative expression level was normalized to values found in control oocytes (right panel). (B) Knockdown efficiency of Abce1 protein was validated by immunostaining of Abce1 (red) and DNA (blue). (C) The kinetics of GVBD was scored at different time points as indicated in the graph, the transition timing of GV to GVBD was measured and shown in the right panel. (D) Characterization of polar body extrusion (PBE) rate after 14 h in culture. A total of 215 oocytes in control and 222 oocytes in Abce1-RNAi were analyzed. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. *p < 0.05 and ***p < 0.001. Scale bar, 20 μm.