Figure 7. CBX7 function was demonstrated in vivo.

(A) LN229/VEC and LN229/CBX7 stabled cells were counted and transplanted subcutaneously in nude mice respectively. After about 4 weeks, xenografted tumors were formed and difference in tumor volume was counted. Size in CBX7 overexpressing groups was smaller than vector groups. (B) Cells were counted and transplanted in orthotopic nude mice model. After about 20 days, HE assay was performed and showed that not only the volume became smaller, but the invasion ability became weak in CBX7 overexpressing groups. Arrow sign points to the invasion sites. (C) CBX7 enhanced survival ability of nude mice injected in orthotopic. (D) CBX7 promote the level of DKK1 in vivo tested by immunohistochemical analysis. (E-G) EMT-like processes were blocked and the expression of MMPs were reduced in vivo, which were analyzed via immunohistochemical analysis.